Mechanical and cell-to-cell adhesive properties of aggregated Methanosarcina

Milkevych, V., Donose, B. C., Juste-Poinapen, N. and Batstone, D. J. (2015) Mechanical and cell-to-cell adhesive properties of aggregated Methanosarcina. Colloids and Surfaces B: Biointerfaces, 126 303-312. doi:10.1016/j.colsurfb.2014.12.035

Author Milkevych, V.
Donose, B. C.
Juste-Poinapen, N.
Batstone, D. J.
Title Mechanical and cell-to-cell adhesive properties of aggregated Methanosarcina
Formatted title
Mechanical and cell-to-cell adhesive properties of aggregated Methanosarcina
Journal name Colloids and Surfaces B: Biointerfaces   Check publisher's open access policy
ISSN 1873-4367
Publication date 2015-02-01
Sub-type Article (original research)
DOI 10.1016/j.colsurfb.2014.12.035
Open Access Status
Volume 126
Start page 303
End page 312
Total pages 10
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2016
Language eng
Formatted abstract
The mechanical and adhesive properties as well as the turgor pressure of microbes play an important role in cell growth and aggregation. By applying AFM together with finite element modelling, one can determine the cell wall structural homogeneity, mechanical and cell-to-cell adhesive properties for aggregated Methanosarcina barkeri cells. This also allows a novel approach to determine in-aggregate turgor pressure determination. Analyzing the AFM force-indentation response of the aggregates under loads less than 10. nN, our study reveals structural inhomogeneity of the polymeric part of the cell wall material and suggests that the cell wall consists of two layers of methanochondroitin (external: with a thickness of 3 ± 1. nm and internal: with a thickness of 169 ± 30. nm). On average, the hyperelastic finite element model showed that the internal layer is more rigid (μ = 14 ± 4. MPa) than the external layer (μ = 2.8 ± 0.9. MPa). To determine the turgor pressure and adhesiveness of the cells, a specific mode of indentation (under a load of 45. nN), aimed towards the centre of the individual aggregate, was performed. By modelling the AFM induced decohesion of the aggregate, the turgor pressure and the cell-to-cell adhesive interface properties could be determined. On average, the turgor pressure is estimated to be 59 ± 22kPa, the interface strength is 78 ± 12. kPa and the polymer network extensibility is 2.8 ± 0.9. nm. We predict that internal cell wall comprised highly compressed methanochondroitin chains and we are able to identify a conceptual model for stress dependent inner cell wall growth.
Keyword Atomic force microscopy
Cell-to-cell interface
Cohesive force
Stress dependent growth
Turgor pressure
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
Advanced Water Management Centre Publications
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