The host metabolite D-serine contributes to bacterial niche specificity through gene selection.

Connolly, James P., Goldstone, Robert J., Burgess, Karl, Cogdell, Richard J., Beatson, Scott A., Vollmer, Waldemar, Smith, David G. E. and Roe, Andrew J. (2014) The host metabolite D-serine contributes to bacterial niche specificity through gene selection.. The ISME Journal, 9 4: 1039-1051. doi:10.1038/ismej.2014.242


Author Connolly, James P.
Goldstone, Robert J.
Burgess, Karl
Cogdell, Richard J.
Beatson, Scott A.
Vollmer, Waldemar
Smith, David G. E.
Roe, Andrew J.
Title The host metabolite D-serine contributes to bacterial niche specificity through gene selection.
Journal name The ISME Journal   Check publisher's open access policy
ISSN 1751-7362
1751-7370
Publication date 2014-12
Year available 2014
Sub-type Article (original research)
DOI 10.1038/ismej.2014.242
Open Access Status
Volume 9
Issue 4
Start page 1039
End page 1051
Total pages 13
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2015
Language eng
Formatted abstract
Escherichia coli comprise a diverse array of both commensals and niche-specific pathotypes. The ability to cause disease results from both carriage of specific virulence factors and regulatory control of these via environmental stimuli. Moreover, host metabolites further refine the response of bacteria to their environment and can dramatically affect the outcome of the host–pathogen interaction. Here, we demonstrate that the host metabolite, D-serine, selectively affects gene expression in E. coli O157:H7. Transcriptomic profiling showed exposure to D-serine results in activation of the SOS response and suppresses expression of the Type 3 Secretion System (T3SS) used to attach to host cells. We also show that concurrent carriage of both the D-serine tolerance locus (dsdCXA) and the locus of enterocyte effacement pathogenicity island encoding a T3SS is extremely rare, a genotype that we attribute to an ‘evolutionary incompatibility’ between the two loci. This study demonstrates the importance of co-operation between both core and pathogenic genetic elements in defining niche specificity.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Chemistry and Molecular Biosciences
 
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