Phosphoinositide 3-kinase p110δ promotes lumen formation through the enhancement of apico-basal polarity and basal membrane organization

Peng, Juan, Awad, Aline, Sar, Sokhavuth, Hamze Komaiha, Ola, Moyano, Romina, Rayal, Amel, Samuel, Didier, Shewan, Annette, Vanhaesebroeck, Bart, Mostov, Keith and Gassama-Diagne, Ama (2015) Phosphoinositide 3-kinase p110δ promotes lumen formation through the enhancement of apico-basal polarity and basal membrane organization. Nature Communications, 6 5937.1-5937.16. doi:10.1038/ncomms6937

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Author Peng, Juan
Awad, Aline
Sar, Sokhavuth
Hamze Komaiha, Ola
Moyano, Romina
Rayal, Amel
Samuel, Didier
Shewan, Annette
Vanhaesebroeck, Bart
Mostov, Keith
Gassama-Diagne, Ama
Title Phosphoinositide 3-kinase p110δ promotes lumen formation through the enhancement of apico-basal polarity and basal membrane organization
Journal name Nature Communications   Check publisher's open access policy
ISSN 2041-1723
Publication date 2015-01-01
Sub-type Article (original research)
DOI 10.1038/ncomms6937
Open Access Status File (Publisher version)
Volume 6
Start page 5937.1
End page 5937.16
Total pages 16
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2016
Language eng
Abstract Signalling triggered by adhesion to the extracellular matrix plays a key role in the spatial orientation of epithelial polarity and formation of lumens in glandular tissues. Phosphoinositide 3-kinase signalling in particular is known to influence the polarization process during epithelial cell morphogenesis. Here, using Madin–Darby canine kidney epithelial cells grown in 3D culture, we show that the p110d isoform of phosphoinositide 3-kinase co-localizes with focal adhesion proteins at the basal surface of polarized cells. Pharmacological, siRNA- or kinase-dead-mediated inhibition of p110d impair the early stages of lumen formation, resulting in inverted polarized cysts, with no laminin or type IV collagen assembly at cell/extracellular matrix contacts. p110d also regulates the organization of focal adhesions and membrane localization of dystroglycan. Thus, we uncover a previously unrecognized role for p110d in epithelial cells in the orientation of the apico-basal axis and lumen formation.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Chemistry and Molecular Biosciences
 
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Citation counts: TR Web of Science Citation Count  Cited 11 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 16 Jan 2015, 21:41:22 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences