Activity-dependent expression of neuronal PAS domain-containing protein 4 (npas4a) in the developing zebrafish brain

Klaric, Thomas, Lardelli, Michael, Key, Brian, Koblar, Simon and Lewis, Martin (2014) Activity-dependent expression of neuronal PAS domain-containing protein 4 (npas4a) in the developing zebrafish brain. Frontiers in Neuroanatomy, 8 148: . doi:10.3389/fnana.2014.00148


Author Klaric, Thomas
Lardelli, Michael
Key, Brian
Koblar, Simon
Lewis, Martin
Title Activity-dependent expression of neuronal PAS domain-containing protein 4 (npas4a) in the developing zebrafish brain
Journal name Frontiers in Neuroanatomy   Check publisher's open access policy
ISSN 1662-5129
Publication date 2014-12-04
Year available 2014
Sub-type Article (original research)
DOI 10.3389/fnana.2014.00148
Open Access Status DOI
Volume 8
Issue 148
Total pages 13
Place of publication Lausanne, Switzerland
Publisher Frontiers Research Foundation
Collection year 2015
Language eng
Formatted abstract
In rodents, the Npas4 gene has recently been identified as being an important regulator of synaptic plasticity and memory. Homologs of Npas4 have been found in invertebrate species though their functions appear to be too divergent for them to be studied as a proxy for the mammalian proteins. The aim of this study, therefore, was to ascertain the suitability of the zebrafish as a model organism for investigating the function of Npas4 genes. We show here that the expression and regulation of the zebrafish Npas4 homolog, npas4a, is remarkably similar to that of the rodent Npas4 genes. As in mammals,
expression of the zebrafish npas4a gene is restricted to the brain where it is up-regulated in response to neuronal activity. Furthermore, we also show that knockdown of npas4a during embryonic development results in a number of forebrain-specific defects including increased apoptosis and misexpression of the forebrain marker genes dlx1a and shha. Our
work demonstrates that the zebrafish is a suitable model organism for investigating the role of the npas4a gene and one that is likely to provide valuable insights into the function of the mammalian homologs. Furthermore, our findings highlight a potential role for npas4a in forebrain development.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Biomedical Sciences Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 3 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 3 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 14 Jan 2015, 17:31:38 EST by Professor Brian Key on behalf of School of Biomedical Sciences