Assessment of lactotroph axis functionality in mice: longitudinal monitoring of PRL secretion by ultrasensitive-ELISA

Guillou, Anne, Romano, Nicola, Steyn, Frederik, Abitbol, Karine, Le Tissier, Paul, Bonnefont, Xavier, Chen, Chen, Mollard, Patrice and Martin, Agnès O. (2015) Assessment of lactotroph axis functionality in mice: longitudinal monitoring of PRL secretion by ultrasensitive-ELISA. Endocrinology, 1-7. doi:10.1210/en.2014-1571

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Author Guillou, Anne
Romano, Nicola
Steyn, Frederik
Abitbol, Karine
Le Tissier, Paul
Bonnefont, Xavier
Chen, Chen
Mollard, Patrice
Martin, Agnès O.
Title Assessment of lactotroph axis functionality in mice: longitudinal monitoring of PRL secretion by ultrasensitive-ELISA
Journal name Endocrinology   Check publisher's open access policy
ISSN 0013-7227
Publication date 2015-02-02
Year available 2015
Sub-type Article (original research)
DOI 10.1210/en.2014-1571
Start page 1
End page 7
Total pages 7
Place of publication Chevy Chase, MD, United States
Publisher The Endocrine Society
Collection year 2016
Language eng
Abstract The pattern of PRL secretion depend on the physiological state. Due to insufficient detection sensitivity of existing assays, the precise description of these patterns in mice is lacking. We described an ultrasensitive ELISA assay that can detect mouse PRL in small fractions of whole blood, allowing longitudinal studies of PRL secretion profiles in freely moving mice. Over a 24h period, males displayed no oscillation in PRL levels while virgin and lactating females showed large pulses. Peaks of PRL secretion reached 30–40ng/mL in lactating female mice, and rarely exceeded 10ng/mL in virgin females. These pulses of PRL in lactating females were associated to suckling. The return of pups after an experimental 12h weaning induced a pulse of PRL release, reaching 100ng/mL. This approach also enabled us to assess the inhibitory tone from hypothalamic dopamine neurons on PRL secretion. We used a dopamine D2 receptor antagonist to relieve pituitary lactotrophs from the tuberoinfundibular dopaminergic (TIDA) inhibitory tone, and demonstrate a D2-induced PRL rise that can be used to evaluate both the secretory capacity of lactotrophs and the magnitude of the inhibitory tone on pituitary PRL release. We demonstrate that whilst lactotroph function is altered to enhance chronic PRL output, their secretory response to acute stimulus is not modified during lactation, and that chronic hyperprolactinemia is linked to a lower inhibitory tone. The combination of a sensitive PRL ELISA and administration of D2 receptor antagonist provide a unique opportunity to investigate the function and plasticity of the lactotroph axis in freely moving mice.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2016 Collection
School of Biomedical Sciences Publications
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Created: Tue, 13 Jan 2015, 16:33:40 EST by Professor Chen Chen on behalf of School of Biomedical Sciences