Molecular analysis of the genera Hapalotrema Looss, 1899 and Learedius Price, 1934 (Digenea: Spirorchiidae) reveals potential cryptic species, with comments on the validity of the genus Learedius

Chapman, Phoebe A., Cribb, Thomas H., Blair, David, Traub, Rebecca J., Kyaw-Tanner, Myat T., Flint, Mark and Mills, Paul C. (2015) Molecular analysis of the genera Hapalotrema Looss, 1899 and Learedius Price, 1934 (Digenea: Spirorchiidae) reveals potential cryptic species, with comments on the validity of the genus Learedius. Systematic Parasitology, 90 1: 67-79. doi:10.1007/s11230-014-9535-y


Author Chapman, Phoebe A.
Cribb, Thomas H.
Blair, David
Traub, Rebecca J.
Kyaw-Tanner, Myat T.
Flint, Mark
Mills, Paul C.
Title Molecular analysis of the genera Hapalotrema Looss, 1899 and Learedius Price, 1934 (Digenea: Spirorchiidae) reveals potential cryptic species, with comments on the validity of the genus Learedius
Journal name Systematic Parasitology   Check publisher's open access policy
ISSN 0165-5752
1573-5192
Publication date 2015
Year available 2015
Sub-type Article (original research)
DOI 10.1007/s11230-014-9535-y
Open Access Status
Volume 90
Issue 1
Start page 67
End page 79
Total pages 13
Place of publication Dordrecht, The Netherlands
Publisher Springer
Collection year 2015
Language eng
Formatted abstract
Adult blood flukes of the genera Hapalotrema Looss, 1899 and Learedius Price, 1934 were collected from turtles off Queensland and the Hawaiian Islands. Specimens were identified as Hapalotrema pambanensis Mehrotra, 1973, H. synorchis Luhman, 1935, H. postorchis Rao, 1976 and Learedius learedi Price, 1934 on the basis of morphology. No major morphological differences were found between specimens from this study and previously published descriptions. DNA was also extracted and sequences obtained using custom spirorchiid-specific primers for the ITS2 and 28S rDNA regions, in order to confirm species identification and investigate phylogenetic relationships. Intraspecific genetic variation was generally low. However the ITS2 region of H. postorchis and to a lesser extent that of L. learedi showed considerable variation between specimens from the Pacific and Atlantic oceans. Further studies will be required to determine whether this variation should be considered inter- or intra-specific. Maximum likelihood phylogenetic analyses were completed for both sequenced genes. Learedius learedi was unequivocally nested among species of Hapalotrema, suggesting that the status of the genus Learedius may need to be reassessed.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Biological Sciences Publications
School of Veterinary Science Publications
 
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