Investigation of a-Phosphoglucomutase from Streptococcus zooepidemicus: Role in Regulation of Polysaccharide Production

Kiriazis, Michael (2001). Investigation of a-Phosphoglucomutase from Streptococcus zooepidemicus: Role in Regulation of Polysaccharide Production Honours Thesis, School of Engineering, The University of Queensland.

       
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Author Kiriazis, Michael
Thesis Title Investigation of a-Phosphoglucomutase from Streptococcus zooepidemicus: Role in Regulation of Polysaccharide Production
School, Centre or Institute School of Engineering
Institution The University of Queensland
Publication date 2001
Thesis type Honours Thesis
Supervisor Lars Nielsen
Total pages 83
Language eng
Subjects 0904 Chemical Engineering
Formatted abstract
This study presents an investigation into the regulatory role that a-PGM has on polymer production (HA) by S. zoo. The growth of L. lactis (strain MG1363), a noneps producing bacteria, was compared to S. zoo, an eps (HA) producing bacteria, were compared with to other. The major difference between the growth of the two bacteria was highlighted by a metabolic flux model to be that the carbon flux from G6P to G1P is much greater in S. zoo, which corresponds to HA production. a-PGM is responsible for the catalysis of this reaction inside the cell. A comparison was made between the activity of this enzyme in crude extracts from each of L. lactis and S. zoo. It was determined that a-PGM shows ten times more activity in S. zoo than in L. lactis, which correlates nicely to the greater flux. Thus a higher activity of a-PGM is potentially responsible for the up regulation of carbon flux towards HA production in S. zoo, and any bacteria engineered to produce HA would need to be equipped with a significantly active a-PGM. Characterisation of the gene from S. zoo encoding a-PGM was started to establish a tool for the up regulation of this important enzyme in any bacteria. However, due to complications and time constraints, the PCR fragment obtained was only partially sequenced. These sequences were compared to S. equi and the alignments provide strong evidence that suggest that the a-PGM gene was found. The results of this study show that a-PGM is a good candidate to be used as a tool to redirect carbon flux towards, and control the rate of, HA production in bacteria.
Keyword a-Phosphoglucomutase

Document type: Thesis
Collection: UQ Theses (non-RHD) - UQ staff and students only
 
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