Amino acid synthesis in the symbiotic sea anemone Aiptasia pulchella

Swanson, R. and Hoegh-Guldberg, O. (1998) Amino acid synthesis in the symbiotic sea anemone Aiptasia pulchella. Marine Biology, 131 1: 83-93.


Author Swanson, R.
Hoegh-Guldberg, O.
Title Amino acid synthesis in the symbiotic sea anemone Aiptasia pulchella
Journal name Marine Biology   Check publisher's open access policy
ISSN 0025-3162
ISBN 0025-3162 (Print) 1432-1793 (Online)
Publication date 1998-04
Sub-type Article (original research)
DOI 10.1007/s002270050299
Volume 131
Issue 1
Start page 83
End page 93
Total pages 11
Place of publication Berlin
Publisher Springer
Language eng
Subject 06 Biological Sciences
Abstract Symbiotic Aiptasia pulchella and freshly isolated zooxanthellae were incubated in (NaHCO3)-C-14 and NH4Cl for 1 to 240 min, and samples were analysed by reverse-phase high-performance liquid chromatography (HPLC) and an online radiochemical detector. NH4+ was first assimilated into C-14-glutamate and C-14-glutamine in the zooxanthellae residing in A. pulchella. The specific activities (dpm nmol(-1)) of C-14-glutamate and C-14-glutamine in vivo, were far greater in the zooxanthellae than in the host tissue, indicating that NH4+ was principally incorporated into the glutamate and glutamine pools of the zooxanthellae. C-14-alpha-ketoglutarate was taken up from the medium by intact A. pulchella and assimilated into a small amount of C-14-glutamate in the host tissue, but no C-14-glutamine was detected in the host fraction. The C-14-glutamate that was synthesized was most likely produced from transamination reactions as opposed to the direct assimilation of NH4+. The free aminoacid composition of the host tissue and zooxanthellae of A. pulchella was also measured. The results presented here demonstrate that NH4+ was initially assimilated by the zooxanthellae of A. pulchella.
Keyword Marine & Freshwater Biology
Dissolved Inorganic Nitrogen
Reef Corals
Glutamate-dehydrogenase
Invertebrate Symbioses
Zooxanthellae
Assimilation
Ammonium
Synthetase
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Centre for Marine Studies Publications
 
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