Enhanced seed oil content by overexpressing genes related to triacylglyceride synthesis

Liu, Fang, Xia, Yuping, Wu, Lei, Fu, Donghui, Hayward, Alice, Luo, Junling, Yan, Xiaohong, Xiong, Xiaojuan, Fu, Ping, Wu, Gang and Lu, Changming (2015) Enhanced seed oil content by overexpressing genes related to triacylglyceride synthesis. Gene, 557 2: 163-171. doi:10.1016/j.gene.2014.12.029


Author Liu, Fang
Xia, Yuping
Wu, Lei
Fu, Donghui
Hayward, Alice
Luo, Junling
Yan, Xiaohong
Xiong, Xiaojuan
Fu, Ping
Wu, Gang
Lu, Changming
Title Enhanced seed oil content by overexpressing genes related to triacylglyceride synthesis
Journal name Gene   Check publisher's open access policy
ISSN 1879-0038
0378-1119
Publication date 2015-02-25
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.gene.2014.12.029
Open Access Status
Volume 557
Issue 2
Start page 163
End page 171
Total pages 9
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2015
Language eng
Formatted abstract
Oilseed rape (Brassica napus) is one of the most important oilseed crops globally. To meet increasing demand for oil-based products, the ability to enhance desirable oil content in the seed is required. This study assessed the capability of five genes in the triacylglyceride (TAG) synthesis pathway to enhance oil content. The genes BnGPDH, BnGPAT, BnDGAT, ScGPDH and ScLPAAT were overexpressed separately in a tobacco (Nicotiana benthamiana) model system, and simultaneously by pyramiding in B. napus, under the control of a seed specific Napin promoter. ScLPAAT transgenic plants showed a significant increase of 6.84% to 8.55% in oil content in tobacco seeds, while a ~ 4% increase was noted for BnGPDH and BnGPAT transgenic seeds. Seed-specific overexpression of all four genes in B. napus resulted in as high a 12.57% to 14.46% increased in seed oil content when compared to WT, equaling close to the sum of the single-gene overexpression increases in tobacco. Taken together, our study demonstrates that BnGPDH, BnGPAT and ScLPAAT may effectively increase seed oil content, and that simultaneous overexpression of these in transgenic B. napus may further enhance the desirable oil content relative to single-gene overexpressors.
Keyword Tobacco
Brassica napus
GPDH
GPAT
DGAT
LPAAT
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available Online 16 December 2014

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2015 Collection
 
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