Temporal renal expression of angiogenic growth factors and their receptors in experimental diabetes: role of the renin-angiotensin system

Rizkalla, Bishoy, Forbes, Josephine M., Cao, Zemin, Boner, Geoffrey and Cooper, Mark E. (2005) Temporal renal expression of angiogenic growth factors and their receptors in experimental diabetes: role of the renin-angiotensin system. Journal of Hypertension, 23 1: 153-164. doi:10.1097/00004872-200501000-00026


Author Rizkalla, Bishoy
Forbes, Josephine M.
Cao, Zemin
Boner, Geoffrey
Cooper, Mark E.
Title Temporal renal expression of angiogenic growth factors and their receptors in experimental diabetes: role of the renin-angiotensin system
Journal name Journal of Hypertension   Check publisher's open access policy
ISSN 0263-6352
1473-5598
Publication date 2005-01
Sub-type Article (original research)
DOI 10.1097/00004872-200501000-00026
Open Access Status Not yet assessed
Volume 23
Issue 1
Start page 153
End page 164
Total pages 12
Place of publication London, United Kingdom
Publisher Lippincott Williams & Wilkins
Language eng
Formatted abstract
Objective: It has been postulated that vascular endothelial growth factor (VEGF) plays a role in the progression of renal injury. However, the role of other angiogenic factors and their receptors, such as the angiopoietins and Tie2, and in particular their relation to renoprotective therapies, such as agents that interrupt the renin-angiotensin system, have not been studied in the context of diabetes-related renal injury.

Design and methods: Renal expression of VEGF, angiopoietin-1 (Ang-1), angiopoietin-2 (Ang-2) and their receptors, VEGF-R2 and Tie-2, were assessed using reverse transcription-polymerase chain reaction, immunohistochemistry and Western blotting, in control and streptozotocin diabetic rats, untreated or receiving the AT1 receptor antagonist, valsartan, or the AT2 receptor antagonist, PD123319.

Results: Diabetes was associated with increased gene and protein expression of VEGF, VEGF-R2, Ang-1, Ang-2 and Tie-2. AT1 receptor antagonism attenuated gene expression of these cytokines and receptors, yet PD123319, which had no effect on blood pressure, reduced VEGF-R2 and Ang-1 gene expression and decreased VEGF, Ang-1 and Ang-2 protein levels.

Conclusions: In experimental diabetes, there is significant upregulatlon within the kidney of various angiogenic cytokines and their receptors. Furthermore, the effects of angiotensin II receptor blockade on these parameters is consistent with the VEGF-VEGF-R2 and angiopoietin - Tie-2 axes being modulated in the kidney by haemodynamic factors in the diabetic context.
Keyword All antagonists
Angiopoietin
Diabetes
Renal disease
Vascular endothelial growth factor
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Mater Research Institute-UQ (MRI-UQ)
 
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