Use of SWATH mass spectrometry for quantitative proteomic investigation of Shewanella oneidensis MR-1 biofilms grown on graphite cloth electrodes

Grobbler, Christy, Virdis, Bernardino, Nouwens, Amanda, Harnisch, Falk, Rabaey, Korneel and Bond, Philip L. (2015) Use of SWATH mass spectrometry for quantitative proteomic investigation of Shewanella oneidensis MR-1 biofilms grown on graphite cloth electrodes. Systematic and Applied Microbiology, 38 2: 135-139. doi:10.1016/j.syapm.2014.11.007

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Author Grobbler, Christy
Virdis, Bernardino
Nouwens, Amanda
Harnisch, Falk
Rabaey, Korneel
Bond, Philip L.
Title Use of SWATH mass spectrometry for quantitative proteomic investigation of Shewanella oneidensis MR-1 biofilms grown on graphite cloth electrodes
Formatted title
Use of SWATH mass spectrometry for quantitative proteomic investigation of Shewanella oneidensis MR-1 biofilms grown on graphite cloth electrodes
Journal name Systematic and Applied Microbiology   Check publisher's open access policy
ISSN 1618-0984
0723-2020
Publication date 2015-03
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.syapm.2014.11.007
Open Access Status
Volume 38
Issue 2
Start page 135
End page 139
Total pages 5
Place of publication Muenchen, Germany
Publisher Elsevier GmbH - Urban und Fischer
Collection year 2015
Language eng
Formatted abstract
Quantitative proteomics from low biomass, biofilm samples is not well documented. In this study we show successful use of SWATH-MS for quantitative proteomic analysis of a microbial electrochemically active biofilm. Shewanella oneidensis MR-1 was grown on carbon cloth electrodes under continuous anodic electrochemical polarizations in a bioelectrochemical system (BES). Using lactate as the electron donor, anodes serving as terminal microbial electron acceptors were operated at three different electrode potentials (+0.71 V, +0.21 V & −0.19 V vs. SHE) and the development of catalytic activity was monitored by measuring the current traces over time. Once maximum current was reached (usually within 21–29 h) the electrochemical systems were shut off and biofilm proteins were extracted from the electrodes for proteomic assessment. SWATH-MS analysis identified 704 proteins, and quantitative comparison was made of those associated with tricarboxcylic acid (TCA) cycle. Metabolic differences detected between the biofilms suggested a branching of the S. oneidensis TCA cycle when grown at the different electrode potentials. In addition, the higher abundance of enzymes involved in the TCA cycle at higher potential indicates an increase in metabolic activity, which is expected given the assumed higher energy gains. This study demonstrates high numbers of identifications on BES biofilm samples can be achieved in comparison to what is currently reported. This is most likely due to the minimal preparation steps required for SWATH-MS.
Keyword Bioelectrochemical system
Electroactive biofilms
Proteomics
Shewanella oneidensis MR1
SWATH-MS
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online ahead of print 29 Nov 2014

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Chemistry and Molecular Biosciences
Advanced Water Management Centre Publications
 
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Created: Tue, 30 Dec 2014, 00:50:00 EST by System User on behalf of Advanced Water Management Centre