The eukaryotic elongation factor 1A is critical for genome replication of the paramyxovirus respiratory syncytial virus

Wei, Ting, Li, Dongsheng, Marcial, Daneth, Khan, Moshin, Lin, Min-Hsuan, Snape, Natale, Ghildyal, Reena, Harrich, David and Spann, Kirsten (2014) The eukaryotic elongation factor 1A is critical for genome replication of the paramyxovirus respiratory syncytial virus. PLoS ONE, 9 12: 1-14. doi:10.1371/journal.pone.0114447


Author Wei, Ting
Li, Dongsheng
Marcial, Daneth
Khan, Moshin
Lin, Min-Hsuan
Snape, Natale
Ghildyal, Reena
Harrich, David
Spann, Kirsten
Title The eukaryotic elongation factor 1A is critical for genome replication of the paramyxovirus respiratory syncytial virus
Journal name PLoS ONE   Check publisher's open access policy
ISSN 1932-6203
Publication date 2014-12-05
Year available 2014
Sub-type Article (original research)
DOI 10.1371/journal.pone.0114447
Open Access Status DOI
Volume 9
Issue 12
Start page 1
End page 14
Total pages 14
Place of publication San Francisco CA, United States
Publisher Public Library of Science
Collection year 2015
Language eng
Abstract The eukaryotic translation factor eEF1A assists replication of many RNA viruses by various mechanisms. Here we show that down-regulation of eEF1A restricts the expression of viral genomic RNA and the release of infectious virus, demonstrating a biological requirement for eEF1A in the respiratory syncytial virus (RSV) life cycle. The key proteins in the replicase/transcriptase complex of RSV; the nucleocapsid (N) protein, phosphoprotein (P) and matrix (M) protein, all associate with eEF1A in RSV infected cells, although N is the strongest binding partner. Using individually expressed proteins, N, but not P or M bound to eEF1A. This study demonstrates a novel interaction between eEF1A and the RSV replication complex, through binding to N protein, to facilitate genomic RNA synthesis and virus production.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Clinical Medical Virology Centre Publications
 
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