3D cultures of prostate cancer cells cultured in a novel high-throughput culture platform are more resistant to chemotherapeutics compared to cells cultured in monolayer

Chambers, Karen F., Mosaad, Eman M. O., Russell, Pamela J., Clements, Judith A. and Doran, Michael R. (2014) 3D cultures of prostate cancer cells cultured in a novel high-throughput culture platform are more resistant to chemotherapeutics compared to cells cultured in monolayer. PLoS One, 9 11: e111029.1-e111029.10. doi:10.1371/journal.pone.0111029


Author Chambers, Karen F.
Mosaad, Eman M. O.
Russell, Pamela J.
Clements, Judith A.
Doran, Michael R.
Title 3D cultures of prostate cancer cells cultured in a novel high-throughput culture platform are more resistant to chemotherapeutics compared to cells cultured in monolayer
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2014-11-07
Year available 2014
Sub-type Article (original research)
DOI 10.1371/journal.pone.0111029
Open Access Status DOI
Volume 9
Issue 11
Start page e111029.1
End page e111029.10
Total pages 10
Place of publication San Francisco, CA United States
Publisher Public Library of Science
Collection year 2015
Language eng
Formatted abstract
Despite monolayer cultures being widely used for cancer drug development and testing, 2D cultures tend to be
hypersensitive to chemotherapy and are relatively poor predictors of whether a drug will provide clinical benefit. Whilst
generally more complicated, three dimensional (3D) culture systems often better recapitulate true cancer architecture and
provide a more accurate drug response. As a step towards making 3D cancer cultures more accessible, we have developed a microwell platform and surface modification protocol to enable high throughput manufacture of 3D cancer aggregates.
Herein we use this novel system to characterize prostate cancer cell microaggregates, including growth kinetics and drug
sensitivity. Our results indicate that prostate cancer cells are viable in this system, however some non-cancerous prostate
cell lines are not. This system allows us to consistently control for the presence or absence of an apoptotic core in the 3D
cancer microaggregates. Similar to tumor tissues, the 3D microaggregates display poor polarity. Critically the response of 3D
microaggregates to the chemotherapeutic drug, docetaxel, is more consistent with in vivo results than the equivalent 2D
controls. Cumulatively, our results demonstrate that these prostate cancer microaggregates better recapitulate the
morphology of prostate tumors compared to 2D and can be used for high-throughput drug testing.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Mater Research Institute-UQ (MRI-UQ)
Official 2015 Collection
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 18 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 20 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 21 Dec 2014, 10:48:24 EST by System User on behalf of Mater Research Institute-UQ