Determination of motility forces on isolated chromosomes with laser tweezers

Khatibzadeh, Nima, Stilgoe, Alexander B., Bui, Ann A. M., Rocha, Yesenia, Cruz, Gladys M., Loke, Vince, Shi, Linda Z., Nieminen, Timo A., Rubinsztein-Dunlop, Halina and Berns, Michael W. (2014) Determination of motility forces on isolated chromosomes with laser tweezers. Scientific Reports, 4 6866: 1-9. doi:10.1038/srep06866


Author Khatibzadeh, Nima
Stilgoe, Alexander B.
Bui, Ann A. M.
Rocha, Yesenia
Cruz, Gladys M.
Loke, Vince
Shi, Linda Z.
Nieminen, Timo A.
Rubinsztein-Dunlop, Halina
Berns, Michael W.
Title Determination of motility forces on isolated chromosomes with laser tweezers
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2014-10-31
Year available 2014
Sub-type Article (original research)
DOI 10.1038/srep06866
Open Access Status DOI
Volume 4
Issue 6866
Start page 1
End page 9
Total pages 9
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2015
Language eng
Abstract Quantitative determination of the motility forces of chromosomes during cell division is fundamental to understanding a process that is universal among eukaryotic organisms. Using an optical tweezers system, isolated mammalian chromosomes were held in a 1064 nm laser trap. The minimum force required to move a single chromosome was determined to be ≈0.8–5 pN. The maximum transverse trapping efficiency of the isolated chromosomes was calculated as ≈0.01–0.02. These results confirm theoretical force calculations of ≈0.1–12 pN to move a chromosome on the mitotic or meiotic spindle. The verification of these results was carried out by calibration of the optical tweezers when trapping microspheres with a diameter of 4.5–15 µm in media with 1–7 cP viscosity. The results of the chromosome and microsphere trapping experiments agree with optical models developed to simulate trapping of cylindrical and spherical specimens.
Keyword Single kinesin molecules
Optical tweezers
Gibberella zeae
Mitotic spindle
Cell membrane
Mitosis
Mechanics
Cytoplasm
Viscosity
Flagella
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Mathematics and Physics
Official 2015 Collection
 
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