Genetic diversity of tick-borne rickettsial pathogens: insights gained from distant strains

Aguilar Pierle, Sebastián, Rosshandler, Ivan Imaz, Akim Kerudin, Ammielle, Sambono, Jacqueline, Lew-Tabor, Ala, Rolls, Peter, Rangel-Escareno, Claudia and Brayton, Kelly A. (2014) Genetic diversity of tick-borne rickettsial pathogens: insights gained from distant strains. Pathogens, 3 1: 57-72. doi:10.3390/pathogens3010057

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Author Aguilar Pierle, Sebastián
Rosshandler, Ivan Imaz
Akim Kerudin, Ammielle
Sambono, Jacqueline
Lew-Tabor, Ala
Rolls, Peter
Rangel-Escareno, Claudia
Brayton, Kelly A.
Title Genetic diversity of tick-borne rickettsial pathogens: insights gained from distant strains
Journal name Pathogens   Check publisher's open access policy
ISSN 2076-0817
Publication date 2014-01-14
Sub-type Article (original research)
DOI 10.3390/pathogens3010057
Open Access Status DOI
Volume 3
Issue 1
Start page 57
End page 72
Total pages 16
Place of publication Basel, Switzerland
Publisher M D P I AG
Collection year 2015
Language eng
Formatted abstract
The ability to capture genetic variation with unprecedented resolution improves our understanding of bacterial populations and their ability to cause disease. The goal of the pathogenomics era is to define genetic diversity that results in disease. Despite the economic losses caused by vector-borne bacteria in the Order Rickettsiales, little is known about the genetic variants responsible for observed phenotypes. The tick-transmitted rickettsial pathogen Anaplasma marginale infects cattle in tropical and subtropical regions worldwide, including Australia. Genomic analysis of North American A. marginale strains reveals a closed core genome defined by high levels of Single Nucleotide Polymorphisms (SNPs). Here we report the first genome sequences and comparative analysis for Australian strains that differ in virulence and transmissibility. A list of genetic differences that segregate with phenotype was evaluated for the ability to distinguish the attenuated strain from virulent field strains. Phylogenetic analyses of the Australian strains revealed a marked evolutionary distance from all previously sequenced strains. SNP analysis showed a strikingly reduced genetic diversity between these strains, with the smallest number of SNPs detected between any two A. marginale strains. The low diversity between these phenotypically distinct bacteria presents a unique opportunity to identify the genetic determinants of virulence and transmission.
Keyword Intracellular bacteria
Comparative genomics
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2015 Collection
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Created: Wed, 17 Dec 2014, 13:24:15 EST by Dr Alicja Lew-tabor on behalf of Centre for Animal Science