Respiratory syncytial virus human experimental infection model: Provenance, production, and sequence of low-Passaged memphis-37 challenge virus

Kim, Young-In, DeVincenzo, John P., Jones, Bart G., Rudraraju, Rajeev, Harrison, Lisa, Meyers, Rachel, Cehelsky, Jeff, Alvarez, Rene and Hurwitz ,Julia L. (2014) Respiratory syncytial virus human experimental infection model: Provenance, production, and sequence of low-Passaged memphis-37 challenge virus. PLoS One, 9 11: . doi:10.1371/journal.pone.0113100


Author Kim, Young-In
DeVincenzo, John P.
Jones, Bart G.
Rudraraju, Rajeev
Harrison, Lisa
Meyers, Rachel
Cehelsky, Jeff
Alvarez, Rene
Hurwitz ,Julia L.
Title Respiratory syncytial virus human experimental infection model: Provenance, production, and sequence of low-Passaged memphis-37 challenge virus
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2014-11-21
Year available 2014
Sub-type Article (original research)
DOI 10.1371/journal.pone.0113100
Open Access Status DOI
Volume 9
Issue 11
Total pages 12
Place of publication San Francisco, CA United States
Publisher Public Library of Science
Collection year 2015
Language eng
Formatted abstract
Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract infections in children and is responsible for as many as 199,000 childhood deaths annually worldwide. To support the development of viral therapeutics and vaccines for RSV, a human adult experimental infection model has been established. In this report, we describe the provenance and sequence of RSV Memphis-37, the low-passage clinical isolate used for the model's reproducible, safe, experimental infections of healthy, adult volunteers. The predicted amino acid sequences for major proteins of Memphis-37 are compared to nine other RSV A and B amino acid sequences to examine sites of vaccine, therapeutic, and pathophysiologic interest. Human T- cell epitope sequences previously defined by in vitro studies were observed to be closely matched between Memphis-37 and the laboratory strain RSV A2. Memphis-37 sequences provide baseline data with which to assess: (i) virus heterogeneity that may be evident following virus infection/transmission, (ii) the efficacy of candidate RSV vaccines and therapeutics in the experimental infection model, and (iii) the potential emergence of escape mutants as a consequence of experimental drug treatments. Memphis-37 is a valuable tool for pre-clinical research, and to expedite the clinical development of vaccines, therapeutic immunomodulatory agents, and other antiviral drug strategies for the protection of vulnerable populations against RSV disease.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
UQ Diamantina Institute Publications
 
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