A protein with protective properties against the cellular defense reactions in insects

Asgari, S, Theopold, U, Wellby, C and Schmidt, O (1998) A protein with protective properties against the cellular defense reactions in insects. Proceedings of The National Academy of Sciences of The United States of America, 95 7: 3690-3695.


Author Asgari, S
Theopold, U
Wellby, C
Schmidt, O
Title A protein with protective properties against the cellular defense reactions in insects
Journal name Proceedings of The National Academy of Sciences of The United States of America   Check publisher's open access policy
Publication date 1998
Sub-type Article
DOI 10.1073/pnas.95.7.3690
Volume number 95
Issue number 7
ISSN 0027-8424
Start page 3690
End page 3695
Total pages 6
Language eng
Abstract The molecular mechanism of how insects recognize intruding microorganisms and parasites and distinguish them from own body structures is not well known. We explored evolutionary adaptations in an insect parasitoid host interaction to identify components that interfere with the recognition of foreign objects and cellular encapsulation. Because some parasitoids provide protection for the developing wasp in the absence of an overt suppression of the insect host defense, we analyzed the surface of eggs and symbiotic viruses for protective properties. Here we report on the molecular cloning of a 32-kDa protein (Crp32) that is one of the major protective components. It is produced in the calyx cells of the female wasp ovaries and attached to the surface of the egg and other particles including polydnaviruses. The recombinant protein confers protection to coated objects in a cellular encapsulation assay suggesting that a layer of Crp32 may prevent cellular encapsulation reactions by a local inactivation of the host defense system.
Keyword Entomology
Immune Recognition
Encapsulation
Calyx Fluid
Cotesia Rubecula
Pieris Rapae
Immunoglobulin Superfamily
Host
Purification
Hemocytes
Hemolin
Cells
Sites
Eggs
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article
Collection: School of Biological Sciences Publications
 
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Created: Mon, 13 Aug 2007, 10:23:41 EST