Ex-post analysis of landraces sympatric to a commercial variety in the center of origin of the potato failed to detect gene flow

Ghislain, M., Montenegro, J. D., Juarez, H. and Herrera, M. D. R. (2014) Ex-post analysis of landraces sympatric to a commercial variety in the center of origin of the potato failed to detect gene flow. Transgenic Research, 24 3: 519-528. doi:10.1007/s11248-014-9854-4


Author Ghislain, M.
Montenegro, J. D.
Juarez, H.
Herrera, M. D. R.
Title Ex-post analysis of landraces sympatric to a commercial variety in the center of origin of the potato failed to detect gene flow
Journal name Transgenic Research   Check publisher's open access policy
ISSN 0962-8819
1573-9368
Publication date 2014-11-29
Year available 2014
Sub-type Article (original research)
DOI 10.1007/s11248-014-9854-4
Open Access Status
Volume 24
Issue 3
Start page 519
End page 528
Total pages 10
Place of publication Dordrecht Netherlands
Publisher Springer Netherlands
Collection year 2015
Formatted abstract
The possible introduction of genetically modified potato in the Andean region raises concerns about the unintentional introduction of transgenes into the native potato germplasm because it is perceived to convey negative impacts on biodiversity. We investigated this question by an ex-post analysis of existing landraces resulting from natural hybridization between an unknown landrace and the fertile commercial variety ‘Yungay’. This variety can be regarded as exotic because it was bred in part from the southern Chilean germplasm of Solanum tuberosum Group Chilotanum. We sampled the landrace germplasm of 1,771 leaf samples comprising more than 400 different landraces from three regions where ‘Yungay’ and landraces have coexisted for 15–25 years in the Peruvian Andes. Simple sequence repeat (SSR) markers were used to identify putative hybrids based on allele sharing with those of ‘Yungay’. The exclusion procedure was iterative, starting with the SSR markers with highest discriminating capacity based on allele frequency of the variety ‘Yungay’ in our large database of 688 landraces by 24 SSR makers. With only 12 of the 24 SSR markers assayed, all of the samples could be rejected as possible hybrids with ‘Yungay’ as a parent. This result demonstrates that the unintentional introduction of a transgene, not under farmers’ selection, from a widely grown transgenic variety over a long period of time is unlikely to happen at a detectable scale. Our finding reinforces the prominent role of farmers in the selection and maintenance of landraces which, unlike hybrids, have specific characteristics that farmers appreciate.
Keyword Transgenic
Gene flow
Center of origin
Potato
Biodiversity
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online ahead of print 29 Nov 2014.

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2015 Collection
 
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