Bacterial expression of two human aryl sulfotransferases

Bidwell, L. M., Gillam, E. M. J., Gaedigk, A., Zhu, X., Grant, D. and McManus, M. E. (1998) Bacterial expression of two human aryl sulfotransferases. Chemico-biological Interactions, 109 1-3: 137-141. doi:10.1016/S0009-2797(97)00128-2

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Author Bidwell, L. M.
Gillam, E. M. J.
Gaedigk, A.
Zhu, X.
Grant, D.
McManus, M. E.
Title Bacterial expression of two human aryl sulfotransferases
Journal name Chemico-biological Interactions   Check publisher's open access policy
ISSN 0009-2797
Publication date 1998-02-20
Sub-type Article (original research)
DOI 10.1016/S0009-2797(97)00128-2
Open Access Status File (Author Post-print)
Volume 109
Issue 1-3
Start page 137
End page 141
Total pages 5
Place of publication Amsterdam
Publisher Elsevier
Language eng
Abstract The effect of replacing a single codon in the N-terminal of human aryl sulfotransferase (HAST) 1 and 3 with one that is more commonly found in E. coli genes was assessed. The pKK233-2 E. coli expression vector was employed and the polymerase chain reaction (PCR) was used to introduce the 5' nucleotide substitution, at the same time maintaining the fidelity of the amino acid sequence. The data indicates that this change had a minimal effect on protein production, subcellular localization or, in the case of HAST3, catalytic activity. In general, the pKK233-2 E. coli vector has been less than optimal for expressing human sulfotransferase cDNAs. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.
Keyword Biochemistry & Molecular Biology
Pharmacology & Pharmacy
Escherichia Coli
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes This is an author version of an article originally published as Bidwell, L. M., Gillam, E. M. J., Gaedigk, A., Zhu, X., Grant, D. and McManus, M. E. (1998) Bacterial expression of two human aryl sulfotransferases, Chemico-biological Interactions 109 (1-3): 137- 141. doi: 10.1016/S0009-2797(97)00128-2 Copyright 1981 Elsevier. All rights reserved. Single copies only may be downloaded and printed for a user’s personal research and study.

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
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Created: Mon, 13 Aug 2007, 10:23:29 EST