Structural insights into the organization of the cavin membrane coat complex

Kovtun, Oleksiy, Tilu, Vikas A., Jung, WooRam, Leneva, Natalya, Ariotti, Nicholas, Chaudhary, Natasha, Mandyam, Ramya A., Ferguson, Charles, Morgan, Garry P., Johnston, Wayne A., Harrop, Stephen J., Alexandrov, Kirill, Parton, Robert G. and Collins, Brett M. (2014) Structural insights into the organization of the cavin membrane coat complex. Developmental Cell, 31 4: 405-419. doi:10.1016/j.devcel.2014.10.002

Author Kovtun, Oleksiy
Tilu, Vikas A.
Jung, WooRam
Leneva, Natalya
Ariotti, Nicholas
Chaudhary, Natasha
Mandyam, Ramya A.
Ferguson, Charles
Morgan, Garry P.
Johnston, Wayne A.
Harrop, Stephen J.
Alexandrov, Kirill
Parton, Robert G.
Collins, Brett M.
Title Structural insights into the organization of the cavin membrane coat complex
Journal name Developmental Cell   Check publisher's open access policy
ISSN 1534-5807
Publication date 2014-11
Sub-type Article (original research)
DOI 10.1016/j.devcel.2014.10.002
Open Access Status
Volume 31
Issue 4
Start page 405
End page 419
Total pages 15
Place of publication Cambridge, MA, United States
Publisher Cell Press
Collection year 2015
Language eng
Abstract Caveolae are cell-surface membrane invaginations that play critical roles in cellular processes including signaling and membrane homeostasis. The cavin proteins, in cooperation with caveolins, are essential for caveola formation. Here we show that a minimal N-terminal domain of the cavins, termed HR1, is required and sufficient for their homo- and hetero-oligomerization. Crystal structures of the mouse cavin1 and zebrafish cavin4a HR1 domains reveal highly conserved trimeric coiled-coil architectures, with intersubunit interactions that determine the specificity of cavin-cavin interactions. The HR1 domain contains a basic surface patch that interacts with polyphosphoinositides and coordinates with additional membrane-binding sites within the cavin C terminus to facilitate membrane association and remodeling. Electron microscopy of purified cavins reveals the existence of large assemblies, composed of a repeating rod-like structural element, and we propose that these structures polymerize through membrane-coupled interactions to form the unique striations observed on the surface of caveolae in vivo.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

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Created: Mon, 15 Dec 2014, 15:36:54 EST by Susan Allen on behalf of Institute for Molecular Bioscience