Advanced glycation end products are direct modulators of β-cell function

Coughlan, Melinda T., Yap, Felicia Y. T., Tong, David C. K., Andrikopoulos, Sofianos, Gasser, Anna, Thallas-Bonke, Vicki, Webster, Diane E., Miyazaki, Jun-ichi, Kay, Thomas W., Slattery, Robyn M., Kaye, David M., Drew, Brian G., Kingwell, Bronwyn A., Fourlanos, Spiros, Groop, Per-Henrik, Harrison, Leonard C., Knip, Mikael and Forbes, Josephine M. (2011) Advanced glycation end products are direct modulators of β-cell function. Diabetes, 60 10: 2523-2532. doi:10.2337/db10-1033


Author Coughlan, Melinda T.
Yap, Felicia Y. T.
Tong, David C. K.
Andrikopoulos, Sofianos
Gasser, Anna
Thallas-Bonke, Vicki
Webster, Diane E.
Miyazaki, Jun-ichi
Kay, Thomas W.
Slattery, Robyn M.
Kaye, David M.
Drew, Brian G.
Kingwell, Bronwyn A.
Fourlanos, Spiros
Groop, Per-Henrik
Harrison, Leonard C.
Knip, Mikael
Forbes, Josephine M.
Title Advanced glycation end products are direct modulators of β-cell function
Journal name Diabetes   Check publisher's open access policy
ISSN 0012-1797
1939-327X
Publication date 2011-10
Sub-type Article (original research)
DOI 10.2337/db10-1033
Open Access Status Not Open Access
Volume 60
Issue 10
Start page 2523
End page 2532
Total pages 10
Place of publication Alexandria, VA United States
Publisher American Diabetes Association
Language eng
Abstract OBJECTIVE - Excess accumulation of advanced glycation end products (AGEs) contributes to aging and chronic diseases. We aimed to obtain evidence that exposure to AGEs plays a role in the development of type 1 diabetes (T1D). RESEARCH DESIGN AND METHODS - The effect of AGEs was examined on insulin secretion by MIN6N8 cells and mouse islets and in vivo in three separate rodent models: AGE-injected or high AGE-fed Sprague-Dawley rats and nonobese diabetic (NODLt) mice. Rodents were also treated with the AGE-lowering agent alagebrium. RESULTS - β-Cells exposed to AGEs displayed acute glucose-stimulated insulin secretory defects, mitochondrial abnormalities including excess superoxide generation, a decline in ATP content, loss of MnSOD activity, reduced calcium flux, and increased glucose uptake, all of which were improved with alagebrium treatment or with MnSOD adenoviral overexpression. Isolated mouse islets exposed to AGEs had decreased glucose-stimulated insulin secretion, increased mitochondrial superoxide production, and depletion of ATP content, which were improved with alagebrium or with MnTBAP, an SOD mimetic. In rats, transient or chronic exposure to AGEs caused progressive insulin secretory defects, superoxide generation, and β-cell death, ameliorated with alagebrium. NODLt mice had increased circulating AGEs in association with an increase in islet mitochondrial superoxide generation, which was prevented by alagebrium, which also reduced the incidence of autoimmune diabetes. Finally, at-risk children who progressed to T1D had higher AGE concentrations than matched nonprogressors. CONCLUSIONS - These findings demonstrate that AGEs directly cause insulin secretory defects, most likely by impairing mitochondrial function, which may contribute to the development of T1D.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Mater Research Institute-UQ (MRI-UQ)
 
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