Characterization and short-term storage of Tasmanian devil sperm collected post-mortem

Keeley, T., McGreevey, P. D. and O'Brien, J. K. (2011) Characterization and short-term storage of Tasmanian devil sperm collected post-mortem. Theriogenology, 76 4: 705-714. doi:10.1016/j.theriogenology.2011.04.001

Author Keeley, T.
McGreevey, P. D.
O'Brien, J. K.
Title Characterization and short-term storage of Tasmanian devil sperm collected post-mortem
Journal name Theriogenology   Check publisher's open access policy
ISSN 0093-691X
Publication date 2011
Sub-type Article (original research)
DOI 10.1016/j.theriogenology.2011.04.001
Open Access Status
Volume 76
Issue 4
Start page 705
End page 714
Total pages 10
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Language eng
Abstract The Tasmanian devil is suffering from a severe population decline due to the fatal Devil Facial Tumour Disease (DFTD). The development of assisted reproductive technologies such as AI and long-term sperm storage could facilitate genetic management of captive insurance populations. The aim of this study was to characterise semen samples collected post-mortem, and to develop a suitable diluent for short-term preservation of devil sperm. Low numbers of sperm (1.33 0.85 10 6 sperm per male) were extracted from the epididymides of 17 males. Devil sperm sample characteristics such as concentration and morphology were similar to other dasyurids. The most commonly observed morphological abnormalities were midpiece separation, tail curling, and tail twisting (on the axial plane). Changes in motility occurred throughout the regions of the epididymis with (mean SD) 29.4 16.8, 46.8 13.6 and 29.4 18.1% of sperm exhibiting motility, and 88.9 11.4, 32.0 24.3 and 0.1 0.2% of motile sperm exhibiting forward progressive motility in the cauda, corpus and caput, respectively. Sperm from the cauda and corpus epididymis maintained 31.7 26.6 and 80.6 85.9%, respectively, of initial motility after 12 h at 15 ;C in a TEST yolk buffer diluent. These findings provided new information regarding devil sperm biology and short-term sperm storage; such information is necessary for future development of long-term sperm preservation methods in the Tasmanian devil.
Keyword Sperm
Tasmanian devil
Gamete Rescue
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
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Created: Mon, 15 Dec 2014, 12:05:49 EST by Tamara Keeley on behalf of School of Agriculture and Food Sciences