Modulation of the cellular expression of circulating advanced glycation end-product receptors in type 2 diabetic nephropathy

Sourris, Karly C., Harcourt, Brooke E., Penfold, Sally A., Yap, Felicia Y. T., Morley, Amy L., Morgan, Philip E., Davies, Michael J., Baker, Scott T., Jerums, George and Forbes, Josephine M. (2010) Modulation of the cellular expression of circulating advanced glycation end-product receptors in type 2 diabetic nephropathy. Experimental Diabetes Research, 2010 Art No.: 974681: . doi:10.1155/2010/974681


Author Sourris, Karly C.
Harcourt, Brooke E.
Penfold, Sally A.
Yap, Felicia Y. T.
Morley, Amy L.
Morgan, Philip E.
Davies, Michael J.
Baker, Scott T.
Jerums, George
Forbes, Josephine M.
Title Modulation of the cellular expression of circulating advanced glycation end-product receptors in type 2 diabetic nephropathy
Journal name Experimental Diabetes Research   Check publisher's open access policy
ISSN 1687-5214
1687-5303
Publication date 2010-01-01
Year available 2010
Sub-type Article (original research)
DOI 10.1155/2010/974681
Open Access Status DOI
Volume 2010
Issue Art No.: 974681
Total pages 9
Place of publication New York, NY United States
Publisher Hindawi Publishing Corporation
Language eng
Formatted abstract
Background. Advanced glycation end-products (AGEs) and their receptors are prominent contributors to diabetic kidney disease. Methods. Flow cytometry was used to measure the predictive capacity for kidney impairment of the AGE receptors RAGE, AGE-R1, and AGE-R3 on peripheral blood mononuclear cells (PBMCs) in experimental models of type 2 diabetes (T2DM) fed varied AGE containing diets and in obese type 2 diabetic and control human subjects.

Results. Diets high in AGE content fed to diabetic mice decreased cell surface RAGE on PBMCs and in type 2 diabetic patients with renal impairment (RI). All diabetic mice had elevated Albumin excretion rates (AERs), and high AGE fed dbdb mice had declining Glomerular filtration rate (GFR). Cell surface AGE-R1 expression was also decreased by high AGE diets and with diabetes in dbdb mice and in humans with RI. PBMC expression of AGE R3 was decreased in diabetic dbdb mice or with a low AGE diet.

Conclusions. The most predictive PBMC profile for renal disease associated with T2DM was an increase in the cell surface expression of AGE-R1, in the context of a decrease in membranous RAGE expression in humans, which warrants further investigation as a biomarker for progressive DN in larger patient cohorts.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Mater Research Institute-UQ (MRI-UQ)
 
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