Insulin infusion reduces hepatocyte growth factor in lean humans

de Courten, Barbora, de Courten, Maximilian P. J., Dougherty, Sonia, Forbes, Josephine M., Potts, Jenna R. and Considine, Robert V. (2013) Insulin infusion reduces hepatocyte growth factor in lean humans. Metabolism: Clinical and Experimental, 62 5: 647-650. doi:10.1016/j.metabol.2012.10.013


Author de Courten, Barbora
de Courten, Maximilian P. J.
Dougherty, Sonia
Forbes, Josephine M.
Potts, Jenna R.
Considine, Robert V.
Title Insulin infusion reduces hepatocyte growth factor in lean humans
Journal name Metabolism: Clinical and Experimental   Check publisher's open access policy
ISSN 0026-0495
1532-8600
Publication date 2013-05
Year available 2012
Sub-type Article (original research)
DOI 10.1016/j.metabol.2012.10.013
Open Access Status
Volume 62
Issue 5
Start page 647
End page 650
Total pages 4
Place of publication Maryland Heights, United States
Publisher W.B. Saunders
Language eng
Formatted abstract
Objective:
Plasma Hepatocyte Growth Factor (HGF) is significantly elevated in obesity and may contribute to vascular disease, metabolic syndrome or cancer in obese individuals. The current studies were done to determine if hyperinsulinemia increases plasma HGF.

Materials/Methods:
Twenty-two participants (10 women/12 men, BMI 20.6-34.5 kg/m2, age 18-49 years) underwent a hyperinsulinemic euglycemic clamp with measurement of HGF at baseline and steady state. Relationships between baseline HGF, anthropometrics, triglycerides, liver enzymes, c-reactive protein and adiponectin were also evaluated.

Results:
Fasting HGF was positively correlated (P < 0.050) with weight (r = 0.63), BMI (r = 0.55), waist circumference (r = 0.68), WHR (r = 0.48), triglycerides (r = 0.44), alanine aminotransferase (r = 0.74) and γ-glutamyl transpeptidase (r = 0.56), but not c-reactive protein or adiponectin. In stepwise regression, alanine aminotransferase and insulin sensitivity accounted for significant variation in fasting HGF. A significant effect of insulin to suppress HGF during the clamp (P = 0.029) was found after adjustment for BMI. HGF was reduced 7% at steady state in the lean subjects only (437.1 ± 57.8 vs 405.4 ± 72.0 pg/ml; P = 0.030).

Conclusions:
The positive correlation of HGF with hepatic enzymes suggests liver may be a significant source of circulating HGF in lean subjects. The strong correlation of plasma HGF with adiposity and the lack of an effect of insulin to increase HGF during the clamp in obese subjects suggest that adiposity, rather than elevated insulin levels, may be the major contributor to plasma HGF in obese subjects. Thus, a reduction in plasma HGF through weight loss is likely the best way to decrease comorbidities mediated by this angiogenic and mitogenic factor.
Keyword Adipose tissue
Hyperinsulinemic-euglycemic clamp
Obesity
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Mater Research Institute-UQ (MRI-UQ)
 
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