Characteristics of primary and immortalized fibroblast cells derived from the miniature and domestic pigs

Oh, Ho-Yeon, Jin, Xun, Kim, Jong-Geun, Oh, Myung-Joo, Pian, Xumin, Kim, Jun-Mo, Yoon, Moon-Seok, Son, Chae-Ik, Lee, Young Sik, Hong, Ki-Chang, Kim, Hyunggee, Choi, Yun-Jaie and Whang, Kwang Youn (2007) Characteristics of primary and immortalized fibroblast cells derived from the miniature and domestic pigs. BMC Cell Biology, 8 . doi:10.1186/1471-2121-8-20


Author Oh, Ho-Yeon
Jin, Xun
Kim, Jong-Geun
Oh, Myung-Joo
Pian, Xumin
Kim, Jun-Mo
Yoon, Moon-Seok
Son, Chae-Ik
Lee, Young Sik
Hong, Ki-Chang
Kim, Hyunggee
Choi, Yun-Jaie
Whang, Kwang Youn
Title Characteristics of primary and immortalized fibroblast cells derived from the miniature and domestic pigs
Journal name BMC Cell Biology   Check publisher's open access policy
ISSN 1471-2121
Publication date 2007-06-01
Sub-type Article (original research)
DOI 10.1186/1471-2121-8-20
Open Access Status DOI
Volume 8
Total pages 1
Language eng
Subject 1307 Cell Biology
Abstract Background: The pig, Sus scrofa domestica includes both the miniature and commercial domestic breed. These animals have influenced the human life and economies and have been studied throughout history. Although the miniature breeds are more recent and have increasingly been used in a variety of biomedical studies, their cell lines have rarely been established. Therefore, we sought to establish primary and immortal cell lines derived from both the miniature and domestic pig to better enable insight into possible in vivo growth differences. Results: The in vitro lifespan of primary domestic pig fibroblast (PF) and miniature pig fibroblast (MPF) cells using a standard 3T3 protocol was determined. Both of the primary PF and MPF cells were shown to have a two-step replicative senescence barrier. Primary MPF cells exhibited a relatively shorter lifespan and slower proliferation rate compared to those of primary PF cells. Beyond senescence barriers, lifespan-extended PF and MPF cells were eventually established and indicated spontaneous cellular immortalization. In contrast to the immortalized PF cells, immortal MPF cells showed a transformed phenotype and possessed more frequent chromosomal abnormalities and loss of p53 regulatory function. The lifespan of primary MPF and PF cells was extended by inactivation of the p53 function using transduction by SV40LT without any detectable senescent phenotype. Conclusion: These results suggest that p53 signaling might be a major determinant for the replicative senescence in the MPF cells that have the shorter lifespan and slower growth rate compared to PF cells in vitro.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
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