A novel pathway of cell death in response to cytosolic DNA in Drosophila cells

Vitak, Nazarii, Johnson, Karyn N., Sester, David P. and Stacey, Katryn J. (2015) A novel pathway of cell death in response to cytosolic DNA in Drosophila cells. Journal of Innate Immunity, 7 2: 212-222. doi:10.1159/000368276

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Author Vitak, Nazarii
Johnson, Karyn N.
Sester, David P.
Stacey, Katryn J.
Title A novel pathway of cell death in response to cytosolic DNA in Drosophila cells
Formatted title
A novel pathway of cell death in response to cytosolic DNA in Drosophila cells
Journal name Journal of Innate Immunity   Check publisher's open access policy
ISSN 1662-811X
Publication date 2015-02
Year available 2014
Sub-type Article (original research)
DOI 10.1159/000368276
Open Access Status File (Author Post-print)
Volume 7
Issue 2
Start page 212
End page 222
Total pages 11
Place of publication Basel, Switzerland
Publisher S. Karger
Collection year 2015
Language eng
Formatted abstract
Defence against invading DNA occurs in both mammals and bacteria. Recognition of stray DNA can initiate responses to infection, but may also protect against potentially mutagenic integration of transposons or retrotransposons into the genome. Double-stranded DNA detected in the cytosol of mammalian macrophages can elicit inflammatory cytokines and cell death following assembly of the AIM2 inflammasome. Amongst eukaryotes, responses to cytosolic DNA have so far only been detected in mammals, and AIM2 is mammalian restricted. In protecting genome integrity, we reasoned that pathways recognising invading DNA should be fundamental to cellular life, and that cell death would be an appropriate response to an overwhelming foreign DNA burden. We found that Drosophila S2 cells were killed by transfection of DNA from a range of natural sources. Unlike with mammalian cells, responses were not prevented by DNA denaturation. There was an element of sequence specificity, as synthetic single-stranded homopolymers were not toxic, whilst mixed-base synthetic DNA caused significant cell death. Death occurred with rapid loss of membrane integrity, and without the characteristic features of apoptosis. We have defined a novel defence against invading DNA in Drosophila. An active necrotic pathway has not previously been described in insects.
Keyword Cytosolic DNA
Cell death
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Biological Sciences Publications
School of Chemistry and Molecular Biosciences
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Citation counts: TR Web of Science Citation Count  Cited 2 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 1 times in Scopus Article | Citations
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Created: Fri, 12 Dec 2014, 10:17:50 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences