Characterization of serum antibodies to Porphyromonas gingivalis in individuals with and without periodontitis

Pietrzak, ER, Polak, B, Walsh, LJ, Savage, NW and Seymour, GJ (1998) Characterization of serum antibodies to Porphyromonas gingivalis in individuals with and without periodontitis. Oral Microbiology And Immunology, 13 2: 65-72. doi:10.1111/j.1399-302X.1998.tb00715.x


Author Pietrzak, ER
Polak, B
Walsh, LJ
Savage, NW
Seymour, GJ
Title Characterization of serum antibodies to Porphyromonas gingivalis in individuals with and without periodontitis
Journal name Oral Microbiology And Immunology   Check publisher's open access policy
ISSN 0902-0055
1399-302X
Publication date 1998-04
Sub-type Article (original research)
DOI 10.1111/j.1399-302X.1998.tb00715.x
Volume 13
Issue 2
Start page 65
End page 72
Total pages 8
Place of publication Oxford, England
Publisher Blackwell Publishing
Language eng
Abstract Although Porphyromonas gingivalis is a defined pathogen in periodontal disease, many subjects control the infection without experiencing loss of attachment. Differences in host susceptibility to the disease may be reflected in the pattern of humoral antibodies against specific P. gingivalis antigens. The aim of this study was to determine the presence of antibodies against immunodominant P. gingivalis antigens as well as the isotype and subclass of anti-P. gingivalis antibodies against outer membrane antigens in four groups of patients: P. gingivalis-positive, 1) with and 2) without periodontitis, and P. gingivalis-negative, 3) with and 4) without periodontitis. Antigens of molecular weight 92, 63, and 32 kDa and lipopolysaccharide were found to be immunodominant. Group 1 subjects showed a significantly higher response to the 92 and 63 kDa antigens compared with other groups. The response to lipopolysaccharide was significantly higher in group 1, and lower in group 4 than in groups 2, 3. Immunoglobulin G(1) (IgG(1)), IgG(2) and IgM antibodies against P. gingivalis outer membrane were present in all subjects, while only some subjects were seropositive for IgG(3), IgG(4) and IgA. There were no differences in concentrations for IgG(1), IgG(3) and IgM. The IgG(2) concentration in group 4 was significantly higher than in groups 1 and 2, while the IgG(4) concentration in group 4 was significantly lower than in other groups. The frequency of seropositivity for IgG(4) and IgA was lowest in group 4, while IgG; seropositivity was almost exclusively seen in healthy patients iii groups 2, 4. These findings suggest that the presence of IgG(3) may reflect non-susceptibility to the disease, while lack of IgG(4) may be indicative of periodontal health and lack of infection.
Keyword Dentistry, Oral Surgery & Medicine
Immunology
Microbiology
Porphyromonas Gingivalis
Antibody
Periodontitis
Immunoglobulin Subclass
P-gingivalis Antigen
Immune Response
Rapidly Progressive Periodontitis
Igg Subclass Distribution
Bacteroides-gingivalis
Secreting Cells
Antigens
Disease
Bacterial
Proteins
Lipopolysaccharide
Identification
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 16 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Mon, 13 Aug 2007, 10:22:19 EST