Production of Alginate Fibres Encapsulating Protein Using a Novel Micro-device Technique

Cameron, Andrew (2006). Production of Alginate Fibres Encapsulating Protein Using a Novel Micro-device Technique Honours Thesis, School of Engineering, The University of Queensland.

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Author Cameron, Andrew
Thesis Title Production of Alginate Fibres Encapsulating Protein Using a Novel Micro-device Technique
School, Centre or Institute School of Engineering
Institution The University of Queensland
Publication date 2006
Thesis type Honours Thesis
Supervisor Justin Cooper-White
Total pages 98
Language eng
Subjects 0904 Chemical Engineering
Formatted abstract
The role of growth factors used in the field of tissue engineering is a very important one. Their ability to control cellular functions such as cell migration, proliferation and differentiation provide a range of opportunities for their application in the area of tissue regeneration. However, the use of growth factors for such applications is hindered by difficulties in their delivery as their efficacy is often both time and dose dependent. Polymeric systems have been proposed as a mode of controlled delivery of growth factors. One such polymeric system is the naturally occurring biopolymer, alginate. Alginate has been used extensively in the field of biotechnology and this study aims to investigate its possible use as a protein delivery system in the form of alginate fibres produced using a novel micro-device technique.

Papain and horseradish peroxidase (HRP) were used as model proteins for the study as they are similar in size to growth factors, they are relatively cheap and their bioactivity can be quantified by coupling them with an appropriate substrate. After sensitivity and degradation analysis, it was found that Papain was not a suitable model protein as it showed a 92% degradation over a period of 22 days when stored at a physiological temperature of 37oC. Therefore HRP was used as the model protein for encapsulation within alginate fibres.

Calcium-alginate fibres encapsulating HRP were produced using a novel polydimethylsiloxane (PDMS) gel micro-device. These fibres were analysed for their encapsulation efficiency and elution characteristics. Results showed the fibres had an encapsulation efficiency between 11.8% and 20.3%. The accumulated elution of the fibres over a 5 day period was measured to be approximately between 32.7% and 34.3%. The results obtained from this study show that alginate fibres produced using the novel micro-device technique could be used for the delivery of growth factors in therapeuticapplications such as wound dressings. This study provides the initial verification that the
novel micro-device fibre fabrication technique is feasible. Future studies can use this information to further investigate its use as method of producing protein delivery systems.
Keyword Alginate Fibres Encapsulating Protein

Document type: Thesis
Collection: UQ Theses (non-RHD) - UQ staff and students only
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Created: Thu, 11 Dec 2014, 11:32:55 EST by Asma Asrar Qureshi on behalf of Scholarly Communication and Digitisation Service