Tailoring strain construction strategies for muconic acid production in S. cerevisiae and E. coli

Averesch, Nils J. H. and Kromer, Jens O. (2014) Tailoring strain construction strategies for muconic acid production in S. cerevisiae and E. coli. Metabolic Engineering Communications, 1 1: 19-28. doi:10.1016/j.meteno.2014.09.001


Author Averesch, Nils J. H.
Kromer, Jens O.
Title Tailoring strain construction strategies for muconic acid production in S. cerevisiae and E. coli
Journal name Metabolic Engineering Communications   Check publisher's open access policy
ISSN 2214-0301
Publication date 2014-10-23
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.meteno.2014.09.001
Open Access Status DOI
Volume 1
Issue 1
Start page 19
End page 28
Total pages 10
Place of publication Amsterdam, The Netherlands
Publisher Elsevier
Collection year 2015
Language eng
Formatted abstract
There is currently a strong interest to derive the biological precursor cis,cis-muconic acid from shikimate pathway-branches to develop a biological replacement for adipic acid. Pioneered by the Frost laboratory this concept has regained interest: Recent approaches (Boles, Alper, Yan) however suffer from low product titres.  Here an in silico comparison of all strain construction strategies was conducted to highlight stoichiometric optimizations. Using elementary mode analysis new knock-out strategies were determined in Saccharomyces cerevisiae and Escherichia coli. The strain construction strategies are unique to each pathway-branch and organism, allowing significantly different maximum and minimum yields. The maximum theoretical product carbon yields on glucose ranged from 86% (dehydroshikimate-branch) to 69% (anthranilate-branch). In most cases a coupling of product formation to growth was possible. Especially in S. cerevisiae chorismate-routes a
minimum yield constraint of 46.9% could be reached. The knock-out targets are non-obvious, and nottransferable, 
highlighting the importance of tailored strain construction strategies
Keyword Elementary mode analysis
Adipic
Muconic
Knock out strategies
Carbon yield
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Chemical Engineering Publications
Official 2015 Collection
Advanced Water Management Centre Publications
 
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Created: Wed, 10 Dec 2014, 15:52:54 EST by Nils Averesch on behalf of School of Chemical Engineering