A highly elastic and adhesive gelatin tissue sealant for gastrointestinal surgery and colon anastomosis

Vuocolo, Tony, Haddad, Roger, Edwards, Glenn A., Lyons, Russell E., Liyou, Nancy E., Werkmeister, Jerome A., Ramshaw, John A. M. and Elvin, Christopher M. (2012) A highly elastic and adhesive gelatin tissue sealant for gastrointestinal surgery and colon anastomosis. Journal of Gastrointestinal Surgery, 16 4: 744-752. doi:10.1007/s11605-011-1771-8


Author Vuocolo, Tony
Haddad, Roger
Edwards, Glenn A.
Lyons, Russell E.
Liyou, Nancy E.
Werkmeister, Jerome A.
Ramshaw, John A. M.
Elvin, Christopher M.
Title A highly elastic and adhesive gelatin tissue sealant for gastrointestinal surgery and colon anastomosis
Journal name Journal of Gastrointestinal Surgery   Check publisher's open access policy
ISSN 1091-255X
1873-4626
Publication date 2012-04
Year available 2011
Sub-type Article (original research)
DOI 10.1007/s11605-011-1771-8
Open Access Status
Volume 16
Issue 4
Start page 744
End page 752
Total pages 9
Place of publication New York, NY, United States
Publisher Springer New York
Collection year 2015
Language eng
Formatted abstract
Background
We describe the development of a highly elastic and adhesive surgical tissue sealant, based on photochemically crosslinked gelatin, for sealing sutured incisions in the gastrointestinal (GI) tract in a rabbit surgical model and in a canine colon anastomosis study.

Methods
The study included in vitro assessment of mechanical parameters of the tissue sealant and in vivo analysis of burst strength and histology at 24 h, 3 days and 7 days post surgery, in a rabbit model, to assess progress of wound healing at the suture sites. Utility of this sealant to repair and seal a lower colonic resection and anastomosis procedure in a canine model was also investigated.

Results
We show that a photopolymerised gelatin tissue sealant provides effective sealing of GI incisions and facilitates wound healing with no evidence of inflammation up to 28 days post-surgery. Blending of derivatised gelatin with underivatised gelatin allowed tuning of elasticity and elastic modulus of the photopolymerised sealant to suit surgical applications. High tissue adhesive strength was maintained at all blend ratios and exceeded 100 kPa.

Conclusions
This highly elastic and adhesive photopolymerised gelatin tissue sealant offers a number of advantages over currently available sealants suitable for GI surgical procedures.
Keyword Tissue sealant
Gelatin
Crosslinking
Photopolymerisation
Gastrointestinal anastomosis
Dehiscence
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Online publication date: 12 Nov 2011

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Veterinary Science Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 11 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 11 times in Scopus Article | Citations
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