Angiotensin-I converting enzyme inhibitory peptides from antihypertensive skate (Okamejei kenojei) skin gelatin hydrolysate in spontaneously hypertensive rats

Ngo, Dai-Hung, Kang, Kyong-Hwa, Ryu, BoMi, Vo, Thanh-Sang, Jung, Won-Kyo, Byun, Hee-Guk and Kim, Se-Kwon (2014) Angiotensin-I converting enzyme inhibitory peptides from antihypertensive skate (Okamejei kenojei) skin gelatin hydrolysate in spontaneously hypertensive rats. Food Chemistry, 174 37-43. doi:10.1016/j.foodchem.2014.11.013


Author Ngo, Dai-Hung
Kang, Kyong-Hwa
Ryu, BoMi
Vo, Thanh-Sang
Jung, Won-Kyo
Byun, Hee-Guk
Kim, Se-Kwon
Title Angiotensin-I converting enzyme inhibitory peptides from antihypertensive skate (Okamejei kenojei) skin gelatin hydrolysate in spontaneously hypertensive rats
Formatted title
Angiotensin-I converting enzyme inhibitory peptides from antihypertensive skate (Okamejei kenojei) skin gelatin hydrolysate in spontaneously hypertensive rats
Journal name Food Chemistry   Check publisher's open access policy
ISSN 1873-7072
0308-8146
Publication date 2014-02-05
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.foodchem.2014.11.013
Open Access Status
Volume 174
Start page 37
End page 43
Total pages 7
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2015
Language eng
Formatted abstract
The aim of this study was to investigate antihypertensive effect of bioactive peptides from skate (Okamejei kenojei) skin gelatin. The Alcalase/protease gelatin hydrolysate below 1 kDa (SAP) exhibited the highest angiotensin-I converting enzyme (ACE) inhibition compared to other hydrolysates. SAP can decrease systolic blood pressure significantly in spontaneously hypertensive rats. SAP inhibited vasoconstriction via PPAR-γ expression, activation and phosphorylation of eNOS in lungs. Moreover, the expression levels of endothelin-1, RhoA, α-smooth muscle actin, cleaved caspase 3 and MAPK were decreased by SAP in lungs. Vascularity, muscularization and cellular proliferation in lungs were detected by immunohistochemical staining. Finally, two purified peptides (LGPLGHQ, 720 Da and MVGSAPGVL, 829 Da) showed potent ACE inhibition with IC50 values of 4.22 and 3.09 μM, respectively. These results indicate that bioactive peptides isolated from skate skin gelatin may serve as candidates against hypertension and could be used as functional food ingredients.
Keyword Skate peptides
Angiotensin converting enzyme inhibition
Antihypertension
Spontaneously hypertensive rats
PPAR-γ-eNOS
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Pharmacy Publications
 
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