Multiphoton microscopy in defining liver function

Thorling, Camilla A., Crawford, Darrell, Burczynski, Frank J., Liu, Xin, Liau, Ian and Roberts, Michael S. (2014) Multiphoton microscopy in defining liver function. Journal of Biomedical Optics, 19 9: . doi:10.1117/1.JBO.19.9.090901


Author Thorling, Camilla A.
Crawford, Darrell
Burczynski, Frank J.
Liu, Xin
Liau, Ian
Roberts, Michael S.
Title Multiphoton microscopy in defining liver function
Journal name Journal of Biomedical Optics   Check publisher's open access policy
ISSN 1083-3668
1560-2281
Publication date 2014-09-01
Year available 2014
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1117/1.JBO.19.9.090901
Open Access Status DOI
Volume 19
Issue 9
Total pages 12
Place of publication Bellingham, WA, United States
Publisher S P I E - International Society for Optical Engineering
Collection year 2015
Language eng
Formatted abstract
Multiphoton microscopy is the preferred method when in vivo deep-tissue imaging is required. This review presents the application of multiphoton microscopy in defining liver function. In particular, multiphoton microscopy is useful in imaging intracellular events, such as mitochondrial depolarization and cellular metabolism in terms of NAD(P)H changes with fluorescence lifetime imaging microscopy. The morphology of hepatocytes can be visualized without exogenously administered fluorescent dyes by utilizing their autofluorescence and second harmonic generation signal of collagen, which is useful in diagnosing liver disease. More specific imaging, such as studying drug transport in normal and diseased livers are achievable, but require exogenously administered fluorescent dyes. If these techniques can be translated into clinical use to assess liver function, it would greatly improve early diagnosis of organ viability, fibrosis, and cancer
Keyword Drug transport
Fibrosis
Fluorescence lifetime imaging microscopy
Hepatocellular Carcinoma
Mitochondria
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Official 2015 Collection
School of Medicine Publications
 
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