Optimization of Cat's Whiskers Tea (Orthosiphon stamineus) using supercritical carbon dioxide and selective chemotherapeutic potential against prostate cancer cells

Al-Suede, Fouad Saleih R., Ahamed, Mohamed B. Khadeer, Majid, Aman S. Abdul, Baharetha, Hussin M., Hassan, Loiy E. A., Kadir, Mohd Omar A., Nassar, Zeyad D. and Majid, Amin M. S. Abdul (2014) Optimization of Cat's Whiskers Tea (Orthosiphon stamineus) using supercritical carbon dioxide and selective chemotherapeutic potential against prostate cancer cells. Evidence-Based Complementary and Alternative Medicine, 2014 396016.1-396016.15. doi:10.1155/2014/396016


Author Al-Suede, Fouad Saleih R.
Ahamed, Mohamed B. Khadeer
Majid, Aman S. Abdul
Baharetha, Hussin M.
Hassan, Loiy E. A.
Kadir, Mohd Omar A.
Nassar, Zeyad D.
Majid, Amin M. S. Abdul
Title Optimization of Cat's Whiskers Tea (Orthosiphon stamineus) using supercritical carbon dioxide and selective chemotherapeutic potential against prostate cancer cells
Formatted title
Optimization of Cat's Whiskers Tea (Orthosiphon stamineus) using supercritical carbon dioxide and selective chemotherapeutic potential against prostate cancer cells
Journal name Evidence-Based Complementary and Alternative Medicine   Check publisher's open access policy
ISSN 1741-427X
1741-4288
Publication date 2014-01
Sub-type Article (original research)
DOI 10.1155/2014/396016
Open Access Status DOI
Volume 2014
Start page 396016.1
End page 396016.15
Total pages 15
Place of publication New York, NY, United States
Publisher Hindawi Publishing Corporation
Collection year 2015
Language eng
Formatted abstract
Cat’s whiskers (Orthosiphon stamineus) leaves extracts were prepared using supercritical CO2 (SC-CO2) with full factorial design to determine the optimum extraction parameters. Nine extracts were obtained by varying pressure, temperature, and time. The extracts were analysed using FTIR, UV-Vis, and GC-MS. Cytotoxicity of the extracts was evaluated on human (colorectal, breast, and prostate) cancer and normal fibroblast cells. Moderate pressure (31.1 MPa) and temperature (60°C) were recorded as optimum extraction conditions with high yield (1.74%) of the extract (B2) at 60 min extraction time. The optimized extract (B2) displayed selective cytotoxicity against prostate cancer (PC3) cells (IC50 28 µg/mL) and significant antioxidant activity (IC50 42.8 µg/mL). Elevated levels of caspases 3/7 and 9 in B2-treated PC3 cells suggest the induction of apoptosis through nuclear and mitochondrial pathways. Hoechst and rhodamine assays confirmed the nuclear condensation and disruption of mitochondrial membrane potential in the cells. B2 also demonstrated inhibitory effects on motility and colonies of PC3 cells at its subcytotoxic concentrations. It is noteworthy that B2 displayed negligible toxicity against the normal cells. Chemometric analysis revealed high content of essential oils, hydrocarbon, fatty acids, esters, and aromatic sesquiterpenes in B2. This study highlights the therapeutic potentials of SC-CO2 extract of cat’s whiskers in targeting prostate carcinoma.
Keyword Fluid Extraction
In-Vitro
Essential Oils
Antioxidant
Apoptosis
Breast
Series
Assays
Co2
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Pharmacy Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in Thomson Reuters Web of Science Article
Scopus Citation Count Cited 4 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 07 Dec 2014, 00:30:41 EST by System User on behalf of School of Pharmacy