Chlamydia trachomatis remodels stable microtubules to coordinate Golgi stack recruitment to the chlamydial inclusion surface

Al-Zeer, Munir A., Al-Youndes, Hesham M., Kerr, Markus, Abu-Lubad, Mohammad, Gonzalez, Erik, Brinkmann, Volker and Meyer, Thomas F. (2014) Chlamydia trachomatis remodels stable microtubules to coordinate Golgi stack recruitment to the chlamydial inclusion surface. Molecular Microbiology, 94 6: 1285-1297. doi:10.1111/mmi.12829


Author Al-Zeer, Munir A.
Al-Youndes, Hesham M.
Kerr, Markus
Abu-Lubad, Mohammad
Gonzalez, Erik
Brinkmann, Volker
Meyer, Thomas F.
Title Chlamydia trachomatis remodels stable microtubules to coordinate Golgi stack recruitment to the chlamydial inclusion surface
Formatted title
Chlamydia trachomatis remodels stable microtubules to coordinate Golgi stack recruitment to the chlamydial inclusion surface
Journal name Molecular Microbiology   Check publisher's open access policy
ISSN 0950-382X
1365-2958
Publication date 2014-10-03
Year available 2014
Sub-type Article (original research)
DOI 10.1111/mmi.12829
Open Access Status
Volume 94
Issue 6
Start page 1285
End page 1297
Total pages 13
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell
Collection year 2015
Language eng
Formatted abstract
Chlamydia trachomatis (Ctr), an obligate intracellular bacterium, survives and replicates within a membrane-bound vacuole, termed the inclusion, which intercepts host exocytic pathways to acquire nutrients. Ctr subverts cellular trafficking pathways from the Golgi by targeting small GTPases, including Rab proteins, to sustain intracellular bacterial replication; however, the precise mechanisms involved remain incompletely understood. Here, we show that Chlamydia infection in human epithelial cells induces microtubule remodeling, in particular the formation of detyrosinated stable MTs, to recruit Golgi ministacks, but not recycling endosomes, to the inclusion. These stable microtubules show increased resistance to chemically induced depolymerization, and their selective depletion results in reduced bacterial infectivity. Rab6 knockdown reversibly prevented not only Golgi ministack formation but also detyrosinated microtubule association with the inclusion. Our data demonstrate that Chlamydia co-opts the function of stable microtubules to support its development.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article first published online: 3 November 2014.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 04 Dec 2014, 10:57:19 EST by Susan Allen on behalf of Institute for Molecular Bioscience