Myostatin is localized in extravillous trophoblast and up-regulates migration

Peiris, Hassendrini N., Salomon, Carlos, Payton, Diane, Ashman, Keith, Vaswani, Kanchan, Chan, Anthony, Rice, Gregory E. and Mitchell, Murray D. (2014) Myostatin is localized in extravillous trophoblast and up-regulates migration. Journal of Clinical Endocrinology and Metabolism, 99 11: E2288-E2297. doi:10.1210/jc.2014-2615

Author Peiris, Hassendrini N.
Salomon, Carlos
Payton, Diane
Ashman, Keith
Vaswani, Kanchan
Chan, Anthony
Rice, Gregory E.
Mitchell, Murray D.
Title Myostatin is localized in extravillous trophoblast and up-regulates migration
Journal name Journal of Clinical Endocrinology and Metabolism   Check publisher's open access policy
ISSN 1945-7197
Publication date 2014-11-01
Year available 2014
Sub-type Article (original research)
DOI 10.1210/jc.2014-2615
Open Access Status
Volume 99
Issue 11
Start page E2288
End page E2297
Total pages 10
Place of publication Chevy Chase, MD United States
Publisher Endocrine Society
Collection year 2015
Language eng
Formatted abstract
Context: Myostatin is a highly conserved secretory protein that negatively regulates muscle development by affecting both proliferation and differentiation of muscle cells. In human placentae the expression of myostatin is negatively correlated with gestational age, and in placental explants, myostatin acts to facilitate glucose uptake. Myostatin expression is known to be higher in the placentae of pregnancies complicated by preeclampsia. Proper placental development is crucial for a healthy and successful pregnancy. Alterations to the function of the placental cells after treatment with myostatin have not previously been published.

Objective: This study investigated the localization of myostatin in extravillous trophoblast (EVT) of human placentae. Furthermore, the effect of myostatin treatment on the proliferative and migrative capabilities of these placental cells was investigated.

Results: Myostatin is localized in EVT, as identified by the immunohistochemistry of third-trimester placentae and immunocytochemistry of first-trimester EVT isolations positively staining for myostatin and human leukocyte antigen-G. Treatment of an EVT cell line (HTR-8/SVneo) and primary isolated EVT with varied concentrations of myostatin resulted in a significant increase in the proliferation (HTR-8/SVneo; P < .0001) and migration (HTR-8/SVneo and primary isolated EVT; P < .05), with proliferation being dose dependent and migration being dose independent.

Conclusions: Myostatin localization was positively identified in EVT. Myostatin positively affected proliferation (HTR-8/SVneo) and migration of EVT (HTR-8/SVneo and primary isolated EVT). For the first time, the effect of myostatin treatment on placental cells is described. The results provide a base from which further in vitro investigations on myostatin's ability to modulate placental cell function can be made.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2015 Collection
School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
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