Control of nutrient solutions for studies at high pH

Kopittke, Peter M. and Menzies, Neal W. (2005) Control of nutrient solutions for studies at high pH. Plant and Soil, 266 1-2: 343-354. doi:10.1007/s11104-005-2658-9

Author Kopittke, Peter M.
Menzies, Neal W.
Title Control of nutrient solutions for studies at high pH
Journal name Plant and Soil   Check publisher's open access policy
ISSN 0032-079X
Publication date 2005-01
Sub-type Article (original research)
DOI 10.1007/s11104-005-2658-9
Volume 266
Issue 1-2
Start page 343
End page 354
Total pages 12
Editor H. Lambers
Place of publication Netherlands
Publisher Springer
Collection year 2004
Language eng
Subject 270400 Botany
Abstract Little is known about factors effecting plant growth at high pH, with research often limited by the inability to separate nutritional deficiencies and HCO3- toxicity from the direct limitations imposed under high pH conditions. Various methods of controlling dilute nutrient solutions for studies at high pH were investigated. For short-term studies, it was found that a solution without Cu, Fe, Mn and Zn and aerated with CO2 depleted air, greatly reduced nutrient precipitation at high pH, thus eliminating nutritional differences between treatments. Manual pH adjustment and the use of ion exchange resins as pH buffers were unsuitable methods of pH control. However, pH control by automated titration had little effect on solution composition while maintaining constant pH. The system described is suitable for studies in which the pH of the bulk nutrient solution must be maintained. The system was used to examine OH- toxicity in mungbeans (Vigna radiata (L.) Wilczek cv. Emerald), with root length reduced at a bulk solution pH of 8.5 and greater.
Keyword alkaline
root growth
seedling nutrient requirement
ion exchange resin
hydroxyl ion
high pH
Q-Index Code C1

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Created: Mon, 06 Aug 2007, 10:26:18 EST by Dr Peter Kopittke on behalf of School of Land, Crop and Food Sciences