MicroRNA biogenesis pathway from the salmon louse (Caligus rogercresseyi): Emerging role in delousing drug response

Valenzuela-Miranda, Diego, Nuñez-Acuña, Gustavo, Valenzuela-Muñoz, Valentina, Asgari, Sassan and Gallardo-Escárate, Cristian (2015) MicroRNA biogenesis pathway from the salmon louse (Caligus rogercresseyi): Emerging role in delousing drug response. Gene, 555 2: 231-241. doi:10.1016/j.gene.2014.11.008


Author Valenzuela-Miranda, Diego
Nuñez-Acuña, Gustavo
Valenzuela-Muñoz, Valentina
Asgari, Sassan
Gallardo-Escárate, Cristian
Title MicroRNA biogenesis pathway from the salmon louse (Caligus rogercresseyi): Emerging role in delousing drug response
Formatted title
MicroRNA biogenesis pathway from the salmon louse (Caligus rogercresseyi): Emerging role in delousing drug response
Journal name Gene   Check publisher's open access policy
ISSN 0378-1119
Publication date 2015-01-25
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.gene.2014.11.008
Open Access Status
Volume 555
Issue 2
Start page 231
End page 241
Total pages 11
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2015
Language eng
Formatted abstract
Despite the increasing evidence of the importance of microRNAs (miRNAs) in the regulation of multiple biological processes, the molecular bases supporting this regulation are still barely understood in crustaceans. Therefore, the molecular characterization and transcriptome modulation of the miRNA biogenesis pathway were evaluated in the salmon louse Caligus rogercresseyi, an ectoparasite that constitutes one of the biggest concerns for salmonid aquaculture industry. Hence, RNA-Seq analysis was conducted from six different developmental stages, and also after bioassays with delousing drugs Deltamethrin and Azamethiphos using adult individuals. In silico analysis evidenced 24 putative genes involved in the miRNA pathway such as biogenesis, transport, maturation and miRNA-target interaction. Moreover, 243 putative single nucleotide polymorphisms (SNPs) were identified, 15 of which showed non-synonym mutations. RNA-Seq analysis revealed that CCR4-Not complex subunit 3 (CNOT3) was upregulated at earlier developmental stages (nauplius I–II and copepodid), and also after the exposure to Azamethiphos, but not to Deltamethrin. In contrast, the subunit 7 (CNOT7) showed an inverse expression pattern. Different Argonaute transcripts were associated to chalimus and adult stages, revealing specific expression patterns in response to antiparasitic drugs. Our results suggest novel insights into the regulatory network of the post-transcriptional gene regulation in C. rogercresseyi mediated by miRNAs, evidencing a putative role during the ontogeny and drug response.
Keyword RNA-Seq
miRNA
Argonaute
Deltamethrin
Azamethiphos
Sea lice
Caligus rogercresseyi
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online: 7 November 2014.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Biological Sciences Publications
 
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Created: Tue, 11 Nov 2014, 20:45:33 EST by Prof Sassan Asgari on behalf of School of Biological Sciences