Ramoplanin at bactericidal concentrations induces bacterial membrane depolarization in Staphylococcus aureus

Cheng, Mu, Huang, Johnny Xiao, Ramu, Soumya, Butler, Mark S. and Cooper, Matthew A. (2014) Ramoplanin at bactericidal concentrations induces bacterial membrane depolarization in Staphylococcus aureus. Antimicrobial Agents and Chemotherapy, 58 11: 6819-6827. doi:10.1128/AAC.00061-14

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Author Cheng, Mu
Huang, Johnny Xiao
Ramu, Soumya
Butler, Mark S.
Cooper, Matthew A.
Title Ramoplanin at bactericidal concentrations induces bacterial membrane depolarization in Staphylococcus aureus
Formatted title
Ramoplanin at bactericidal concentrations induces bacterial membrane depolarization in Staphylococcus aureus
Journal name Antimicrobial Agents and Chemotherapy   Check publisher's open access policy
ISSN 1098-6596
0066-4804
Publication date 2014-11-01
Sub-type Article (original research)
DOI 10.1128/AAC.00061-14
Open Access Status File (Publisher version)
Volume 58
Issue 11
Start page 6819
End page 6827
Total pages 9
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2015
Language eng
Formatted abstract
Ramoplanin is an actinomycetes-derived antibiotic with broad-spectrum activity against Gram-positive bacteria that has been evaluated in clinical trials for the treatment of gastrointestinal vancomycin-resistant enterococci (VRE) and Clostridium difficile infections. Recent studies have proposed that ramoplanin binds to bacterial membranes as a C2 symmetrical dimer that can sequester Lipid II, which causes inhibition of cell wall peptidoglycan biosynthesis and cell death. In this study, ramoplanin was shown to bind to anionic and zwitterionic membrane mimetics with a higher affinity for anionic membranes and to induce membrane depolarization of methicillin-susceptible Staphylococcus aureus (MSSA) ATCC 25923 at concentrations at or above the minimal bactericidal concentration (MBC). The ultrastructural effects of ramoplanin on S. aureus were also examined by transmission electron microscopy (TEM), and this showed dramatic changes to bacterial cell morphology. The correlation observed between membrane depolarization and bacterial cell viability suggests that this mechanism may contribute to the bactericidal activity of ramoplanin.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Institute for Molecular Bioscience - Publications
 
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