West Nile virus (WNV) infection alters the cytokines and TLRs transcriptome profiles in rabbit PBL

Uddin, Jasim M., Suen, Willy, Prow, Natalie A, Hall, Roy A. and Bielefeldt-Ohmann, Helle (2014). West Nile virus (WNV) infection alters the cytokines and TLRs transcriptome profiles in rabbit PBL. In: SI: 2014 ICIS Abstract Issue, ICIS Conference. 2nd Annual Meeting of the International Cytokine and Interferon Society (ICIS), Melbourne, VIC Australia, (73-73). 26 - 29 October 2014. doi:10.1016/j.cyto.2014.07.194


Author Uddin, Jasim M.
Suen, Willy
Prow, Natalie A
Hall, Roy A.
Bielefeldt-Ohmann, Helle
Title of paper West Nile virus (WNV) infection alters the cytokines and TLRs transcriptome profiles in rabbit PBL
Conference name 2nd Annual Meeting of the International Cytokine and Interferon Society (ICIS)
Conference location Melbourne, VIC Australia
Conference dates 26 - 29 October 2014
Proceedings title SI: 2014 ICIS Abstract Issue, ICIS Conference   Check publisher's open access policy
Journal name Cytokine   Check publisher's open access policy
Place of Publication London, United Kingdom
Publisher Academic Press
Publication Year 2014
Year available 2014
Sub-type Published abstract
DOI 10.1016/j.cyto.2014.07.194
Open Access Status
ISSN 1043-4666
1096-0023
Volume 70
Issue 1
Start page 73
End page 73
Total pages 1
Collection year 2015
Language eng
Formatted Abstract/Summary
The peripheral innate immune response to WNV is crucial for control of virus spread to the brain. Therefore, transcriptomes involved in the innate immune response against WNV were investigated in a model organism of rabbit peripheral blood mononuclear leukocytes (PBLs). PBLs were challenged with WNVNSW2011in vitro and mRNA expressions were quantified at 2 h, 6 h, 12 h and 24 h post challenge (pc) using qRT-PCR. Compared to mock infected PBL, WNV infected PBLs expressed high levels of IFNα at 6 h pc. IFNγ expression increased markedly, peaking at 12 h pc with a 4-fold increase. IL6, 12 & 22 expression peaked at 12 h pc; while CXCL10 and PTX3 expression were higher at 2 h pc and then declined. TLR1, 2, 3, 4, 6 & 10 were up-regulated at 2 h pc with highest expression seen for TLR3, 4 & 6. TLRs-associated downstream genes (MyD88, STAT1, TRAF3, IRF7 & IRF9) were up-regulated between 6 and 24 h pc. Higher expression of TRAF3, STAT1, IRF7 & IRF9 suggest the activation of TLR signaling pathways in order to detect and clear the virus. Higher expression of TNFα, HO1, iNOS, Caspase3 & Caspase9 transcripts suggest the involvement of oxidative stress and apoptosis pathway in WNV infected rabbit PBLs. The expression dynamics of selected genes were validated in PBLs from rabbits experimentally infected with WNV in vivo. Higher expression of IFNα, IFNβ, IFNγ, TNFα, IL6, IL22, PTX3, TLR3 & TLR4, IRF7 & IRF9, STST1, TRAF3, Caspase3 & Caspase9 were found in PBL from WNV infected rabbits compared to PBLs from un-infected control rabbits, and coincided with expression patterns of these genes in vitro. WNVNSW2011 gene expression increased over time in PBLs challenged in vitro but remained undetected in PBLs from in vivo infected and control rabbits. This study highlights the array of cytokines and TLRs involved in the host innate immune response to WNV.
Q-Index Code EX
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Abstract 187

 
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Created: Fri, 07 Nov 2014, 10:28:19 EST by Mrs Louise Nimwegen on behalf of School of Veterinary Science