The effect of riboflavin and ultraviolet light on the infectivity of arboviruses

Faddy, Helen M., Prow, Natalie A., Fryk, Jesse J., Hall, Roy A., Keil, Shawn D., Goodrich, Raymond P. and Marks, Denese C. (2014) The effect of riboflavin and ultraviolet light on the infectivity of arboviruses. Transfusion, 55 4: 824-831. doi:10.1111/trf.12899

Author Faddy, Helen M.
Prow, Natalie A.
Fryk, Jesse J.
Hall, Roy A.
Keil, Shawn D.
Goodrich, Raymond P.
Marks, Denese C.
Title The effect of riboflavin and ultraviolet light on the infectivity of arboviruses
Journal name Transfusion   Check publisher's open access policy
ISSN 0041-1132
Publication date 2014-11
Year available 2014
Sub-type Article (original research)
DOI 10.1111/trf.12899
Open Access Status
Volume 55
Issue 4
Start page 824
End page 831
Total pages 8
Place of publication Hoboken, NJ United States
Publisher Wiley-Blackwell Publishing, Inc.
Collection year 2015
Language eng
Formatted abstract
Arboviruses are an emerging threat to transfusion safety and rates of infection are likely to increase with the increased rainfall associated with climate change. Arboviral infections are common in Australia, where Ross River virus (RRV), Barmah Forest virus (BFV), and Murray Valley encephalitis virus (MVEV), among others, have the potential to cause disease in humans. The use of pathogen reduction technology (PRT) may be an alternative approach for blood services to manage the risk of arboviral transfusion transmission. In this study, the effectiveness of the Mirasol PRT (Terumo BCT) system at inactivating RRV, BFV, and MVEV in buffy coat (BC)-derived platelets (PLTs) was investigated.

Study Design and Methods
BC-derived PLT concentrates in additive solution (SSP+) were spiked with RRV, BFV, or MVEV and then treated with the Mirasol PRT system. The level of infectious virus was determined before and after treatment, and the reduction in viral infectivity was calculated.

Treatment with PRT (Mirasol) reduced the amount of infectious virus of all three arboviruses. The greatest level of inactivation was observed for RRV (2.33 log; 99.25%), followed by BFV (1.97 log; 98.68%) and then MVEV (1.83 log; 98.42%).

Our study demonstrates that treatment of PLT concentrates with PRT (Mirasol) reduces the infectious levels of RRV, BFV, and MVEV. The relevance of the level of reduction required to prevent disease transmission by transfusion has not been fully defined and requires further investigation. In the face of a changing climate, with its associated threat to blood safety, PRT represents a proactive approach for maintaining blood safety.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Chemistry and Molecular Biosciences
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 6 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 4 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 07 Nov 2014, 10:26:06 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences