Effect of surface functionality of silica nanoparticles on cellular uptake and cytotoxicity

Jambhrunkar, Siddharth, Qu, Zhi, Popat, Amirali, Yang, Jie, Noonan, Owen, Acauan, Luiz, Ahmad Nor, Yusilawati, Yu, Chengzhong and Karmakar, Surajit (2014) Effect of surface functionality of silica nanoparticles on cellular uptake and cytotoxicity. Molecular Pharmaceutics, 11 10: 3642-3655. doi:10.1021/mp500385n


Author Jambhrunkar, Siddharth
Qu, Zhi
Popat, Amirali
Yang, Jie
Noonan, Owen
Acauan, Luiz
Ahmad Nor, Yusilawati
Yu, Chengzhong
Karmakar, Surajit
Title Effect of surface functionality of silica nanoparticles on cellular uptake and cytotoxicity
Journal name Molecular Pharmaceutics   Check publisher's open access policy
ISSN 1543-8392
1543-8384
Publication date 2014-10-06
Year available 2014
Sub-type Article (original research)
DOI 10.1021/mp500385n
Open Access Status
Volume 11
Issue 10
Start page 3642
End page 3655
Total pages 14
Place of publication Washingtons, DC, United States
Publisher American Chemical Society
Collection year 2015
Language eng
Abstract Mesoporous silica nanoparticles (MCM-41) with different surface chemistry were used as carrier system to study its influence on drug delivery and anticancer activity of curcumin (CUR). CUR was encapsulated in pristine MCM-41 (hydrophilic and negatively charged), amino functionalized MCM-41 (MCM-41-NH2 which is hydrophilic and positively charged), and methyl functionalized MCM-41 (MCM-41-CH3 which is hydrophobic and negatively charged) and evaluated for in vitro release and cell cytotoxicity in human squamous cell carcinoma cell line (SCC25). Various techniques were employed to evaluate the performance of these materials on cellular uptake and anticancer activity in the SCC25 cell line. Both positively and negatively charged surfaces demonstrated enhanced drug release and anticancer activity compared to pure CUR. Positively charged nanoparticles showed higher cell uptake compared to negatively charged nanoparticles owing to its electrostatic interaction with cells. However, hydrophobic surface modified nanoparticles (MCM-41-CH3) showed no improvement in drug release and anticancer activity due to its poor wetting effect. Cell cycle analysis and cell apoptosis studies revealed different pathway mechanisms followed by the positively and negatively charged nanoparticles but exhibiting similar anticancer activity in SCC25 cells.
Keyword Mesoporous silica
Curcumin
Surface charge
Hydrophobicity
In vitro release
Cell cycle
Cell apoptosis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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