BraLTP1, a lipid transfer protein gene involved in epicuticular wax deposition, cell proliferation and flower development in Brassica napus

Liu, Fang, Xiong, Xiaojuan, Wu, Lei, Fu, Donghui, Hayward, Alice, Zeng, Xinhua, Cao, Yinglong, Wu, Yuhua, Li, Yunjing and Wu, Gang (2014) BraLTP1, a lipid transfer protein gene involved in epicuticular wax deposition, cell proliferation and flower development in Brassica napus. PLoS ONE, 9 10: 1-12. doi:10.1371/journal.pone.0110272


Author Liu, Fang
Xiong, Xiaojuan
Wu, Lei
Fu, Donghui
Hayward, Alice
Zeng, Xinhua
Cao, Yinglong
Wu, Yuhua
Li, Yunjing
Wu, Gang
Title BraLTP1, a lipid transfer protein gene involved in epicuticular wax deposition, cell proliferation and flower development in Brassica napus
Journal name PLoS ONE   Check publisher's open access policy
ISSN 1932-6203
Publication date 2014-10-14
Year available 2014
Sub-type Article (original research)
DOI 10.1371/journal.pone.0110272
Open Access Status DOI
Volume 9
Issue 10
Start page 1
End page 12
Total pages 12
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Collection year 2015
Language eng
Abstract Plant non-specific lipid transfer proteins (nsLTPs) constitute large multigene families that possess complex physiological functions, many of which remain unclear. This study isolated and characterized the function of a lipid transfer protein gene, BraLTP1 from Brassica rapa, in the important oilseed crops Brassica napus. BraLTP1 encodes a predicted secretory protein, in the little known VI Class of nsLTP families. Overexpression of BnaLTP1 in B. napus caused abnormal green coloration and reduced wax deposition on leaves and detailed wax analysis revealed 17–80% reduction in various major wax components, which resulted in significant water-loss relative to wild type. BnaLTP1 overexpressing leaves exhibited morphological disfiguration and abaxially curled leaf edges, and leaf cross-sections revealed cell overproliferation that was correlated to increased cytokinin levels (tZ, tZR, iP, and iPR) in leaves and high expression of the cytokinin biosynthsis gene IPT3. BnaLTP1-overexpressing plants also displayed morphological disfiguration of flowers, with early-onset and elongated carpel development and outwardly curled stamen. This was consistent with altered expression of a a number of ABC model genes related to flower development. Together, these results suggest that BraLTP1 is a new nsLTP gene involved in wax production or deposition, with additional direct or indirect effects on cell division and flower development.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2015 Collection
 
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