Exhaustion of the chloroplast protein synthesis capacity by massive expression of a highly stable protein antibiotic

Oey, Melanie, Lohse, Marc, Kreikemeyer, Bernd and Bock, Ralph (2008) Exhaustion of the chloroplast protein synthesis capacity by massive expression of a highly stable protein antibiotic. Plant Journal, 57 3: 436-445. doi:10.1111/j.1365-313X.2008.03702.x


Author Oey, Melanie
Lohse, Marc
Kreikemeyer, Bernd
Bock, Ralph
Title Exhaustion of the chloroplast protein synthesis capacity by massive expression of a highly stable protein antibiotic
Journal name Plant Journal   Check publisher's open access policy
ISSN 0960-7412
1365-313X
Publication date 2008-10-30
Year available 2008
Sub-type Article (original research)
DOI 10.1111/j.1365-313X.2008.03702.x
Open Access Status
Volume 57
Issue 3
Start page 436
End page 445
Total pages 10
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing Ltd.
Collection year 2008
Language eng
Formatted abstract
Plastids (chloroplasts) possess an enormous capacity to synthesize and accumulate foreign proteins. Here we have maximized chloroplast protein production by over-expressing a proteinaceous antibiotic against pathogenic group A and group B streptococci from the plastid genome. The antibiotic, a phage lytic protein, accumulated to enormously high levels (>70% of the plant’s total soluble protein), and proved to be extremely stable in chloroplasts. This massive over-expression exhausted the protein synthesis capacity of the chloroplast such that the production of endogenous plastid-encoded proteins was severely compromised. Our data suggest that this is due to translational rather than transcriptional limitation of gene expression. We also show that the chloroplast-produced protein antibiotic efficiently kills the target bacteria. These unrivaled expression levels, together with the chloroplast’s insensitivity to enzymes that degrade bacterial cell walls and the elimination of the need to remove bacterial endotoxins by costly purification procedures, indicate that this is an effective plant-based production platform for next-generation antibiotics, which are urgently required to keep pace with rapidly emerging bacterial resistance.
Keyword Chloroplast
Plastid transformation
Translation
Protein Stability
Molecular farming
Protein antibiotic
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 138 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 148 times in Scopus Article | Citations
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Created: Wed, 29 Oct 2014, 07:24:17 EST by Melanie Oey on behalf of Institute for Molecular Bioscience