Generalized platform for antibody detection using the antibody catalyzed water oxidation pathway

Welch, M. Elizabeth, Ritzert, Nicole L., Chen, Hongjun, Smith, Norah L., Tague, Michele E., Xu, Youyong, Baird, Barbara A., Abruna, Hector D. and Ober, Christopher K. (2014) Generalized platform for antibody detection using the antibody catalyzed water oxidation pathway. Journal of the American Chemical Society, 136 5: 1879-1883. doi:10.1021/ja409598c


Author Welch, M. Elizabeth
Ritzert, Nicole L.
Chen, Hongjun
Smith, Norah L.
Tague, Michele E.
Xu, Youyong
Baird, Barbara A.
Abruna, Hector D.
Ober, Christopher K.
Title Generalized platform for antibody detection using the antibody catalyzed water oxidation pathway
Journal name Journal of the American Chemical Society   Check publisher's open access policy
ISSN 0002-7863
1520-5126
Publication date 2014-02-05
Sub-type Article (original research)
DOI 10.1021/ja409598c
Open Access Status DOI
Volume 136
Issue 5
Start page 1879
End page 1883
Total pages 5
Place of publication Washington DC United States
Publisher American Chemical Society
Collection year 2015
Language eng
Subject 1600 Chemistry
Abstract Infectious diseases, such as influenza, present a prominent global problem including the constant threat of pandemics that initiate in avian or other species and then pass to humans. We report a new sensor that can be specifically functionalized to detect antibodies associated with a wide range of infectious diseases in multiple species. This biosensor is based on electrochemical detection of hydrogen peroxide generated through the intrinsic catalytic activity of all antibodies: the antibody catalyzed water oxidation pathway (ACWOP). Our platform includes a polymer brush-modified surface where specific antibodies bind to conjugated haptens with high affinity and specificity. Hydrogen peroxide provides an electrochemical signal that is mediated by Resorufin/Amplex Red. We characterize the biosensor platform, using model anti-DNP antibodies, with the ultimate goal of designing a versatile device that is inexpensive, portable, reliable, and fast. We demonstrate detection of antibodies at concentrations that fall well within clinically relevant levels.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Chemical Engineering Publications
Non HERDC
 
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