Electrochemical surface plasmon resonance detection of enzymatic reaction in bilayer lipid membranes

Wang, Jianlong, Wang, Fuan, Chen, Hongjun, Liu, Xiaohua and Dong, Shaojun (2008) Electrochemical surface plasmon resonance detection of enzymatic reaction in bilayer lipid membranes. Talanta, 75 3: 666-670. doi:10.1016/j.talanta.2007.11.062


Author Wang, Jianlong
Wang, Fuan
Chen, Hongjun
Liu, Xiaohua
Dong, Shaojun
Title Electrochemical surface plasmon resonance detection of enzymatic reaction in bilayer lipid membranes
Journal name Talanta   Check publisher's open access policy
ISSN 0039-9140
1873-3573
Publication date 2008-05
Year available 2007
Sub-type Article (original research)
DOI 10.1016/j.talanta.2007.11.062
Open Access Status
Volume 75
Issue 3
Start page 666
End page 670
Total pages 5
Place of publication Amsterdam Netherlands
Publisher Elsevier BV
Language eng
Formatted abstract
In this paper, electrochemical surface plasmon resonance (SPR) method was first used to detect enzymatic reaction in bilayer lipid membrane (BLM) based on immobilizing horseradish peroxidase (HRP) in the BLMs supported by the redox polyaniline (PAn) film. By SPR kinetic curve in situ monitoring the redox transformation of PAn film resulted from the reaction between HRP and PAn, the enzymatic reaction of HRP with H2O2 was successfully analyzed by electrochemical SPR spectroscopy. The results show that this BLM supported on PAn film cannot only preserve the bioactivity of HRP immobilized in the membrane, but also provide a channel for the transfer of electrons between HRP and PAn on electrode surface. These characteristics enabled the development of SPR biosensor for sensitively detecting H2O2. H2O2 has been detected by electrochemical SPR spectroscopy in the concentration range of 5 × 10-5 M to 2 × 10-3 M. After each of detections, the SPR sensor surface was completely regenerated by electrochemically reducing the oxidized PAn to its reduced state. This method provides a novel route for enhancing the detection of small ligand of enzymatic reaction in BLM by electrochemical SPR spectroscopy.
Keyword Electrochemical surface plasmon resonance
Enzymatic reaction
LBM
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemical Engineering Publications
 
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