Direct electron transfer and electrocatalysis of microperoxidase immobilized on nanohybrid film

Liu, Ying, Wang, Mingkui, Zhao, Feng, Guo, Zhihui, Chen, Hongjun and Dong, Shaojun (2005) Direct electron transfer and electrocatalysis of microperoxidase immobilized on nanohybrid film. Journal of Electroanalytical Chemistry, 581 1: 1-10. doi:10.1016/j.jelechem.2005.01.022


Author Liu, Ying
Wang, Mingkui
Zhao, Feng
Guo, Zhihui
Chen, Hongjun
Dong, Shaojun
Title Direct electron transfer and electrocatalysis of microperoxidase immobilized on nanohybrid film
Journal name Journal of Electroanalytical Chemistry   Check publisher's open access policy
ISSN 0022-0728
1873-2569
Publication date 2005-07-15
Sub-type Article (original research)
DOI 10.1016/j.jelechem.2005.01.022
Open Access Status Not yet assessed
Volume 581
Issue 1
Start page 1
End page 10
Total pages 10
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Language eng
Formatted abstract
The nanohybrid film (NHF) consisted of multi-wall carbon nanotubes (MWTNs) with attached gold nanoparticles was used to immobilize protein. The NHF provides a favorable microenvironment for microperoxidase (MP-11) to perform direct electron transfer (DET) at glassy carbon electrode. The NHF and MP-11 attached covalently were confirmed by transmission electron microscopy, atomic force microscopy and X-ray photoelectron spectroscopy. MP-11 immobilized on NHF surface exhibits a surface-controlled quasi-reversible cyclic voltammetric behavior. The electrochemical parameters such as apparent heterogeneous electron rate constant (k), formal potential (E0′) and the influence of pH on E0′ in the process were estimated by cyclic voltammetry. The MP-11 immobilized on the NHF retains its bioelectrocatalytic activity to the reduction of H2O2. The apparent Michaelis-Menten constant (Km) and stability of the H2O2 response were investigated. Moreover, it can catalyze O2 through four-electron to water in pH 7.0 buffer solution. The kinetic constants for O2 reduction (ks, αnα) were obtained. The NHF to immobilize protein with DET shows potential applicability of nanofabrication of biosensors and biofuel cells.
Keyword Carbon nanotubes
Electrocatalysis
Electron transfer
Gold nanoparticles
Microperoxidase-11
Nanohybrid film
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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