Spatiotemporal regulation of multiple overlapping sense and novel natural antisense transcripts at the nrgn and camk2n1 gene loci during mouse cerebral corticogenesis

Ling, King-Hwa, Hewitt, Chelsee A., Beissbarth, Tim, Hyde, Lavinia, Cheah, Pike-See, Smyth, Gordon K., Tan, Seong-Seng, Hahn, Christopher N., Thomas, Tim, Thomas, Paul Q. and Scott, Hamish S. (2011) Spatiotemporal regulation of multiple overlapping sense and novel natural antisense transcripts at the nrgn and camk2n1 gene loci during mouse cerebral corticogenesis. Cerebral Cortex, 21 3: 683-697. doi:10.1093/cercor/bhq141


Author Ling, King-Hwa
Hewitt, Chelsee A.
Beissbarth, Tim
Hyde, Lavinia
Cheah, Pike-See
Smyth, Gordon K.
Tan, Seong-Seng
Hahn, Christopher N.
Thomas, Tim
Thomas, Paul Q.
Scott, Hamish S.
Title Spatiotemporal regulation of multiple overlapping sense and novel natural antisense transcripts at the nrgn and camk2n1 gene loci during mouse cerebral corticogenesis
Journal name Cerebral Cortex   Check publisher's open access policy
ISSN 1047-3211
1460-2199
Publication date 2011
Year available 2011
Sub-type Article (original research)
DOI 10.1093/cercor/bhq141
Open Access Status
Volume 21
Issue 3
Start page 683
End page 697
Total pages 15
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Collection year 2011
Language eng
Abstract Nrgn and Camk2n1 are highly expressed in the brain and play an important role in synaptic long-term potentiation via regulation of Ca2 +/calmodulin-dependent protein kinase II. We have shown that the gene loci for these 2 proteins are actively transcribed in the adult cerebral cortex and feature multiple overlapping transcripts in both the sense and antisense orientations with alternative polyadenylation. These transcripts were upregulated in the adult compared with embryonic and P1.5 mouse cerebral cortices, and transcripts with different 3′ untranslated region lengths showed differing expression profiles. In situ hybridization (ISH) analysis revealed spatiotemporal regulation of the Nrgn and Camk2n1 sense and natural antisense transcripts (NATs) throughout cerebral corticogenesis. In addition, we also demonstrated that the expression of these transcripts was organ-specific. Both Nrgn and Camk2n1 sense and NATs were also upregulated in differentiating P19 teratocarcinoma cells. RNA fluorescent ISH analysis confirmed the capability of these NATs to form double-stranded RNA aggregates with the sense transcripts in the cytoplasm of cells obtained from the brain. We propose that the differential regulation of multiple sense and novel overlapping NATs at the Nrgn and Camk2n1 loci will increase the diversity of posttranscriptional regulation, resulting in cell- and time-specific regulation of their gene products during cerebral corticogenesis and function.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
 
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Created: Mon, 27 Oct 2014, 17:11:21 EST by Sylvie Pichelin on behalf of Queensland Brain Institute