Amino acid signatures in the developing mouse retina

Nivison-Smith, Lisa, Chua, Jacqueline, Tan, Seong-Seng and Kalloniatis, Michael (2014) Amino acid signatures in the developing mouse retina. International Journal of Developmental Neuroscience, 33 1: 62-80. doi:10.1016/j.ijdevneu.2013.12.005


Author Nivison-Smith, Lisa
Chua, Jacqueline
Tan, Seong-Seng
Kalloniatis, Michael
Title Amino acid signatures in the developing mouse retina
Journal name International Journal of Developmental Neuroscience   Check publisher's open access policy
ISSN 0736-5748
1873-474X
Publication date 2014
Sub-type Article (original research)
DOI 10.1016/j.ijdevneu.2013.12.005
Open Access Status
Volume 33
Issue 1
Start page 62
End page 80
Total pages 19
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon Press
Collection year 2015
Abstract This study characterizes the developmental patterns of seven key amino acids: glutamate, γ-amino-butyric acid (GABA), glycine, glutamine, aspartate, alanine and taurine in the mouse retina. We analyze amino acids in specific bipolar, amacrine and ganglion cell sub-populations (i.e. GABAergic vs. glycinergic amacrine cells) and anatomically distinct regions of photoreceptors and Müller cells (i.e. cell bodies vs. endfeet) by extracting data from previously described pattern recognition analysis. Pattern recognition statistically classifies all cells in the retina based on their neurochemical profile and surpasses the previous limitations of anatomical and morphological identification of cells in the immature retina. We found that the GABA and glycine cellular content reached adult-like levels in most neurons before glutamate. The metabolic amino acids glutamine, aspartate and alanine also reached maturity in most retinal cells before eye opening. When the overall amino acid profiles were considered for each cell group, ganglion cells and GABAergic amacrine cells matured first, followed by glycinergic amacrine cells and finally bipolar cells. Photoreceptor cell bodies reached adult-like amino acid profiles at P7 whilst Müller cells acquired typical amino acid profiles in their cell bodies at P7 and in their endfeet by P14. We further compared the amino acid profiles of the C57Bl/6J mouse with the transgenic X-inactivation mouse carrying the lacZ gene on the X chromosome and validated this animal model for the study of normal retinal development. This study provides valuable insight into normal retinal neurochemical maturation and metabolism and benchmark amino acid values for comparison with retinal disease, particularly those which occur during development.
Keyword Alanine
Amino acids
Aspartate
GABA
Glutamate
Glutamine
Glycine
Immunocytochemistry
Retina development
Taurine
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Queensland Brain Institute Publications
 
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Created: Mon, 27 Oct 2014, 17:04:33 EST by Sylvie Pichelin on behalf of Queensland Brain Institute