The precerebellar linear nucleus in the mouse defined by connections, immunohistochemistry, and gene expression

Fu, YuHong, Turdik, Petr, Makki, Nadja, Palombi, Olivier, Machold, Robert, Paxinos, George and Watson, Charles (2009) The precerebellar linear nucleus in the mouse defined by connections, immunohistochemistry, and gene expression. Brain Research, 1271 49-59. doi:10.1016/j.brainres.2009.02.068


Author Fu, YuHong
Turdik, Petr
Makki, Nadja
Palombi, Olivier
Machold, Robert
Paxinos, George
Watson, Charles
Title The precerebellar linear nucleus in the mouse defined by connections, immunohistochemistry, and gene expression
Journal name Brain Research   Check publisher's open access policy
ISSN 0006-8993
1872-6240
Publication date 2009-05-19
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.brainres.2009.02.068
Open Access Status
Volume 1271
Start page 49
End page 59
Total pages 11
Place of publication Amsterdam, The Netherlands
Publisher Elsevier BV
Collection year 2009
Language eng
Abstract The linear nucleus (Li) is a prominent cell group in the caudal hindbrain, which was first described in a study of cerebellar afferents in the rat by [Watson, C.R.R., Switzer, R.C. III, 1978. Trigeminal projections to cerebellar tactile areas in the rat origin mainly from N. interpolaris and N. principalis. Neurosci. Lett. 10, 77-82.]. It was named for its elongated appearance in transverse sections. Since this original description in the rat, reference to the nucleus seems to have been largely absent from experimental studies of mammalian precerebellar nuclei. We therefore set out to define the cytoarchitecture, cerebellar connections, and molecular characteristics of Li in the mouse. In coronal Nissl sections at the level of the rostral inferior olive, it consists of two parallel bands of cells joined at their dorsal apex by a further band of cells, making the shape of the Greek capital letter pi. Our three-dimensional reconstruction demonstrated that the nucleus is continuous with the lateral reticular nucleus (LRt) and that the ambiguus nucleus sits inside the arch of Li. Cerebellar horseradish peroxidase injections confirmed that the cells of Li project to cerebellum. We have shown that Li cells express Atoh1 and Wnt1 lineage markers that are known to label the rhombic lip derived precerebellar nuclei. We have examined the relationship of Li cells to a number of molecular markers, and have found that many of the cells express a nonphosphorylated epitope in neurofilament H (SMI 32), a feature they share with the LRt. The mouse Li therefore appears to be a rostrodorsal extension of the LRt.
Keyword Lateral reticular nucleus
Linear nucleus
Precerebellar nucleus
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
 
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Created: Mon, 27 Oct 2014, 16:40:07 EST by Sylvie Pichelin on behalf of Queensland Brain Institute