Short-coherence off-axis holographic phase microscopy of live cell dynamics

Witte, Stefan, Plauska, Andrius, Ridder, Margreet C., van Berge, Laura, Mansvelder, Huibert D. and Groot, Marie Louise (2012) Short-coherence off-axis holographic phase microscopy of live cell dynamics. Biomedical Optics Express, 3 9: 2184-2189. doi:10.1364/BOE.3.002184


Author Witte, Stefan
Plauska, Andrius
Ridder, Margreet C.
van Berge, Laura
Mansvelder, Huibert D.
Groot, Marie Louise
Title Short-coherence off-axis holographic phase microscopy of live cell dynamics
Journal name Biomedical Optics Express   Check publisher's open access policy
ISSN 2156-7085
Publication date 2012
Sub-type Article (original research)
DOI 10.1364/BOE.3.002184
Open Access Status DOI
Volume 3
Issue 9
Start page 2184
End page 2189
Total pages 6
Place of publication Washington, DC, United States
Publisher Optical Society of America
Language eng
Abstract We demonstrate a single-shot holographic phase microscope that combines short-coherence laser pulses with an off-axis geometry. By introducing a controlled pulse front tilt, ultrashort pulses are made to interfere over a large field-of-view without loss of fringe contrast. With this microscope, quantitative phase images of live cells can be recorded in a fullfield geometry without moving parts. We perform phase imaging of HEK293 cells, to study the dynamics of cell volume regulation in response to an osmotic shock.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
 
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Created: Mon, 27 Oct 2014, 15:31:56 EST by Debra McMurtrie on behalf of Queensland Brain Institute